BMRB Entry 4127
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4127
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Title: Sequence-specific 1H, 13C, and 15N Resonance Assignment of the Origin DNA-binding Domain of SV40 T Antigen
Deposition date: 1998-03-20 Original release date: 1999-03-04
Authors: Luo, Xuelian; Sanford, David; Bullock, Peter; Bachovchin, William
Citation: Luo, Xuelian; Sanford, David; Bullock, Peter; Bachovchin, William. "Solution Structure of the Origin DNA-binding Domain of SV40 T-antigen" Nat. Struct. Biol. 3, 1034-1039 (1996).
Assembly members:
Origin DNA-binding domain of SV40 T-antigen, polymer, 134 residues, 15390 Da.
Natural source: Common Name: simian virus 40 Taxonomy ID: 10633 Superkingdom: not available Kingdom: not available Genus/species: Polyomavirus Simian virus 40
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Origin DNA-binding domain of SV40 T-antigen: GSKVEDPKDFPSELLSFLSH
AVFSNRTLACFAIYTTKEKA
ALLYKKIMEKYSVTFISRHN
SYNHNILFFLTPHRHRVSAI
NNYAQKLCTFSFLICKGVNK
EYLMYSALTRDPFSVIEESL
PGGLKEHDFNPESS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 475 |
| 15N chemical shifts | 130 |
| 1H chemical shifts | 885 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | T-Ag_OBD | 1 |
Entities:
Entity 1, T-Ag_OBD 134 residues - 15390 Da.
| 1 | GLY | SER | LYS | VAL | GLU | ASP | PRO | LYS | ASP | PHE | ||||
| 2 | PRO | SER | GLU | LEU | LEU | SER | PHE | LEU | SER | HIS | ||||
| 3 | ALA | VAL | PHE | SER | ASN | ARG | THR | LEU | ALA | CYS | ||||
| 4 | PHE | ALA | ILE | TYR | THR | THR | LYS | GLU | LYS | ALA | ||||
| 5 | ALA | LEU | LEU | TYR | LYS | LYS | ILE | MET | GLU | LYS | ||||
| 6 | TYR | SER | VAL | THR | PHE | ILE | SER | ARG | HIS | ASN | ||||
| 7 | SER | TYR | ASN | HIS | ASN | ILE | LEU | PHE | PHE | LEU | ||||
| 8 | THR | PRO | HIS | ARG | HIS | ARG | VAL | SER | ALA | ILE | ||||
| 9 | ASN | ASN | TYR | ALA | GLN | LYS | LEU | CYS | THR | PHE | ||||
| 10 | SER | PHE | LEU | ILE | CYS | LYS | GLY | VAL | ASN | LYS | ||||
| 11 | GLU | TYR | LEU | MET | TYR | SER | ALA | LEU | THR | ARG | ||||
| 12 | ASP | PRO | PHE | SER | VAL | ILE | GLU | GLU | SER | LEU | ||||
| 13 | PRO | GLY | GLY | LEU | LYS | GLU | HIS | ASP | PHE | ASN | ||||
| 14 | PRO | GLU | SER | SER |
Samples:
sample_one: Origin DNA-binding domain of SV40 T-antigen, [U-15N;U-13C], 3 mM
sample_conditions_one: pH: 5.5 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| PDB | |
| EMBL | CAB82893 CBL79143 |
| GB | AAB59924 AAC59346 AAD38846 AAD38993 AAD39001 |
| REF | YP_003708382 |
| SP | P03070 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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