BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4560

Title: Assignment of 1H, 13C and 15N signals of a recombinant effector protein (T4MOD) in Toluene-4-monooxygenase complex

Deposition date: 1999-12-13 Original release date: 2000-04-03

Authors: Hemmi, Hikaru; Chae, Young Kee; Studts, Joey; Fox, Brian; Markley, John

Citation: Hemmi, Hikaru; Chae, Young; Studts, Joey; Fox, Brian; Markley, John. "Letter to the Editor: Assignment of 1H, 13C and 15N signals of a recombinant effector protein (T4MOD) in Toluene-4-monooxygenase complex"  J. Biomol. NMR 16, 359-360 (2000).

Assembly members:
T4MOD, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: Pseudomonas mendocina   Taxonomy ID: 300   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas mendocina

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
T4MOD: STLADQALHNNNVGPIIRAG DLVEPVIETAEIDNPGKEIT VEDRRAYVRIAAEGELILTR KTLEEQLGRPFNMQELEINL ASFAGQIQADEDQIRFYFDK TM

Data sets:
Data typeCount
1H chemical shifts730
13C chemical shifts458
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1T4MOD1

Entities:

Entity 1, T4MOD 102 residues - Formula weight is not available

1   SERTHRLEUALAASPGLNALALEUHISASN
2   ASNASNVALGLYPROILEILEARGALAGLY
3   ASPLEUVALGLUPROVALILEGLUTHRALA
4   GLUILEASPASNPROGLYLYSGLUILETHR
5   VALGLUASPARGARGALATYRVALARGILE
6   ALAALAGLUGLYGLULEUILELEUTHRARG
7   LYSTHRLEUGLUGLUGLNLEUGLYARGPRO
8   PHEASNMETGLNGLULEUGLUILEASNLEU
9   ALASERPHEALAGLYGLNILEGLNALAASP
10   GLUASPGLNILEARGPHETYRPHEASPLYS
11   THRMET

Samples:

sample_1: T4MOD, [U-13C; U-15N], 1.1 mM

sample_2: T4MOD2.5 – 3.5 mM

cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D CCONHnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D HCCONHnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
3D CT-13C-HSQCnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 13C-1H-1H NOESYnot availablenot availablenot available
3D 15N TOCSY-HSQCnot availablenot availablenot available

Software:

FELIX v95 - processing of NMR data

Sparky - assignment

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
GB AAA26002 AAS66663 AJO68018
SP Q00459

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts