BMRB Entry 4675

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4675

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 13C and 15N resonance assignments of the DNA binding domain of the human forkhead transcription factor AFX

Deposition date: 2000-03-01 Original release date: 2000-07-08

Authors: Weigelt, Johan; Climent, Isabel; Dahlman-Wright, Karin; Wikstrom, Mats

Citation: Weigelt, Johan; Climent, Isabel; Dahlman-Wright, Karin; Wikstrom, Mats. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the DNA binding domain of the human forkhead transcription factor AFX" J. Biomol. NMR 17, 181-182 (2000).

Assembly members:
AFX, polymer, 150 residues, 16708 Da.

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
AFX: GSSHHHHHHSSGLVPRGSHM LEDPGAVTGPRKGGSRRNAW GNQSYAELISQAIESAPEKR LTLAQIYEWMVRTVPYFKDK GDSNSSAGWKNSIRHNLSLH SKFIKVHNEATGKSSWWMLN PEGGKSGKAPRRRAASMDSS SKLLRGRSKA

Data sets:

assigned_chemical_shifts
- Chemical_shifts

Data type	Count
15N chemical shifts	139
1H chemical shifts	529
13C chemical shifts	494

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AFX-DBD	1

Entities:

Entity 1, AFX-DBD 150 residues - 16708 Da.

1	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS	SER
2	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS	MET
3	LEU	GLU	ASP	PRO	GLY	ALA	VAL	THR	GLY	PRO
4	ARG	LYS	GLY	GLY	SER	ARG	ARG	ASN	ALA	TRP
5	GLY	ASN	GLN	SER	TYR	ALA	GLU	LEU	ILE	SER
6	GLN	ALA	ILE	GLU	SER	ALA	PRO	GLU	LYS	ARG
7	LEU	THR	LEU	ALA	GLN	ILE	TYR	GLU	TRP	MET
8	VAL	ARG	THR	VAL	PRO	TYR	PHE	LYS	ASP	LYS
9	GLY	ASP	SER	ASN	SER	SER	ALA	GLY	TRP	LYS
10	ASN	SER	ILE	ARG	HIS	ASN	LEU	SER	LEU	HIS
11	SER	LYS	PHE	ILE	LYS	VAL	HIS	ASN	GLU	ALA
12	THR	GLY	LYS	SER	SER	TRP	TRP	MET	LEU	ASN
13	PRO	GLU	GLY	GLY	LYS	SER	GLY	LYS	ALA	PRO
14	ARG	ARG	ARG	ALA	ALA	SER	MET	ASP	SER	SER
15	SER	LYS	LEU	LEU	ARG	GLY	ARG	SER	LYS	ALA

Samples:

sample_1: AFX, [U-13C; U-15N], 1.5 mM; Na phosphate 20 mM; NaCl 100 mM; EDTA 1 mM; Pefabloc 1 mM; NaN3 0.02 % w/v

conditions_1: pH: 6.3; temperature: 304 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian Unity Inova 800 MHz

PDB	1E17 3L2C
DBJ	BAA86199 BAG61928 BAG73163
EMBL	CAA63819 CAA72156
GB	AAD42108 AAI06762 AAI39276 AAI39305 AAI48035
REF	NP_001094747 NP_005929 NP_061259 XP_002720130 XP_002763017
SP	P98177 Q9WVH3
TPG	DAA12928

Biological Magnetic Resonance Data Bank