BMRB Entry 4717
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4717
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Title: 1H, 15N, 13C, and 13CO Assignments for ZipA
Deposition date: 2000-04-11 Original release date: 2000-05-24
Authors: Moy, Franklin; Glasfeld, Elizabeth; Powers, Robert
Citation: Moy, Franklin; Glasfeld, Elizabeth; Powers, Robert. "Letter to the Editor: 1H, 15N, 13C, and 13CO assignments and secondary structure determination of ZipA*" J. Biomol. NMR 17, 275-276 (2000).
Assembly members:
E. coli ZipA, polymer, 144 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
E. coli ZipA: MDKPKRKEAVIIMNVAAHHG
SELNGELLLNSIQQAGFIFG
DMNIYHRHLSPDGSGPALFS
LANMVKPGTFDPEMKDFTTP
GVTIFMQVPSYGDELQNFKL
MLQSAQHIADEVGGVVLDDQ
RRMMTPQKLREYQDIIREVK
DANA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 925 |
| 13C chemical shifts | 611 |
| 15N chemical shifts | 136 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ZipA | 1 |
Entities:
Entity 1, ZipA 144 residues - Formula weight is not available
| 1 | MET | ASP | LYS | PRO | LYS | ARG | LYS | GLU | ALA | VAL | ||||
| 2 | ILE | ILE | MET | ASN | VAL | ALA | ALA | HIS | HIS | GLY | ||||
| 3 | SER | GLU | LEU | ASN | GLY | GLU | LEU | LEU | LEU | ASN | ||||
| 4 | SER | ILE | GLN | GLN | ALA | GLY | PHE | ILE | PHE | GLY | ||||
| 5 | ASP | MET | ASN | ILE | TYR | HIS | ARG | HIS | LEU | SER | ||||
| 6 | PRO | ASP | GLY | SER | GLY | PRO | ALA | LEU | PHE | SER | ||||
| 7 | LEU | ALA | ASN | MET | VAL | LYS | PRO | GLY | THR | PHE | ||||
| 8 | ASP | PRO | GLU | MET | LYS | ASP | PHE | THR | THR | PRO | ||||
| 9 | GLY | VAL | THR | ILE | PHE | MET | GLN | VAL | PRO | SER | ||||
| 10 | TYR | GLY | ASP | GLU | LEU | GLN | ASN | PHE | LYS | LEU | ||||
| 11 | MET | LEU | GLN | SER | ALA | GLN | HIS | ILE | ALA | ASP | ||||
| 12 | GLU | VAL | GLY | GLY | VAL | VAL | LEU | ASP | ASP | GLN | ||||
| 13 | ARG | ARG | MET | MET | THR | PRO | GLN | LYS | LEU | ARG | ||||
| 14 | GLU | TYR | GLN | ASP | ILE | ILE | ARG | GLU | VAL | LYS | ||||
| 15 | ASP | ALA | ASN | ALA |
Samples:
sample_1: E. coli ZipA, [U-15N], 1.0 mM; potassium phosphate 50 mM; NaN3 2 mM; DTT, [U-2H], 50 mM; H2O 90%; D2O 10%
sample_2: E. coli ZipA, [U-13C; U-15N], 1.0 mM; potassium phosphate 50 mM; NaN3 2 mM; DTT, [U-2H], 50 mM; H2O 90%; D2O 10%
Ex-cond_1: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA16284 BAB36707 BAG78227 BAI26665 BAI31697 |
| EMBL | CAP76884 CAQ32790 CAQ99310 CAR03874 CAR09031 |
| GB | AAB42061 AAC75465 AAG57531 AAN43974 AAN81396 |
| REF | NP_311311 NP_416907 NP_708267 WP_000314370 WP_000983142 |
| SP | A1ADS9 A7ZPL3 A8A2Q8 B1IX59 B1LMK6 |
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