BMRB Entry 4719
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4719
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Title: Backbone 1H, 13C and 15N chemical shift assignments of the Ras-binding domain (RBD) of AF6
Deposition date: 2000-04-12 Original release date: 2001-03-15
Authors: Steiner, Guido; Kremer, Werner; Linnemann, Thomas; Herrmann, Christian; Geyer, Matthias; Kalbitzer, Hans
Citation: Steiner, Guido; Kremer, Werner; Linnemann, Thomas; Herrmann, Christian; Geyer, Matthias; Kalbitzer, Hans. "Sequence-specific Resonance Assignment of the Ras-binding Domain of AF6" J. Biomol. NMR 18, 73-74 (2000).
Assembly members:
rnAF6, polymer, 141 residues, 16481 Da.
Natural source: Common Name: rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norwegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
rnAF6: MSAGGRDEERRKLADIIHHW
NANRLDLFEISQPTEDLEFH
GVMRFYFQDKAAGNFATKCI
RVSSTATTQDVIETLAEKFR
PDMRMLSSPKYSLYEVHVSG
ERRLDIDEKPLVVQLNWNKD
DREGRFVLKNENDAIPAKKA
Q
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 483 |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 754 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AF6 | 1 |
Entities:
Entity 1, AF6 141 residues - 16481 Da.
| 1 | MET | SER | ALA | GLY | GLY | ARG | ASP | GLU | GLU | ARG | ||||
| 2 | ARG | LYS | LEU | ALA | ASP | ILE | ILE | HIS | HIS | TRP | ||||
| 3 | ASN | ALA | ASN | ARG | LEU | ASP | LEU | PHE | GLU | ILE | ||||
| 4 | SER | GLN | PRO | THR | GLU | ASP | LEU | GLU | PHE | HIS | ||||
| 5 | GLY | VAL | MET | ARG | PHE | TYR | PHE | GLN | ASP | LYS | ||||
| 6 | ALA | ALA | GLY | ASN | PHE | ALA | THR | LYS | CYS | ILE | ||||
| 7 | ARG | VAL | SER | SER | THR | ALA | THR | THR | GLN | ASP | ||||
| 8 | VAL | ILE | GLU | THR | LEU | ALA | GLU | LYS | PHE | ARG | ||||
| 9 | PRO | ASP | MET | ARG | MET | LEU | SER | SER | PRO | LYS | ||||
| 10 | TYR | SER | LEU | TYR | GLU | VAL | HIS | VAL | SER | GLY | ||||
| 11 | GLU | ARG | ARG | LEU | ASP | ILE | ASP | GLU | LYS | PRO | ||||
| 12 | LEU | VAL | VAL | GLN | LEU | ASN | TRP | ASN | LYS | ASP | ||||
| 13 | ASP | ARG | GLU | GLY | ARG | PHE | VAL | LEU | LYS | ASN | ||||
| 14 | GLU | ASN | ASP | ALA | ILE | PRO | ALA | LYS | LYS | ALA | ||||
| 15 | GLN |
Samples:
unlabeled_protein: rnAF6 0.9 mM
15N_labeled_protein: rnAF6, [U-15N], 1.3 mM
13C_15N_labeled_protein: rnAF6, [U-13C; U-15N], 1.0 – 1.3 mM
standard_conditions: pH: 6.9; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| CBCANH | not available | not available | not available |
| 15N-TOCSY-HSQC | not available | not available | not available |
| 15N-NOESY-HSQC | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 13C-NOESY-HSQC | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| DBJ | BAA32483 BAA32484 BAA32485 BAD92657 BAE38754 |
| GB | AAC50059 AAC53390 AAC53391 AAD54283 AAI56049 |
| REF | NP_001035089 NP_001193937 NP_001278893 NP_034936 NP_037349 |
| SP | O35889 P55196 Q9QZQ1 |
| TPG | DAA25947 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts