BMRB Entry 4773
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4773
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Title: Backbone assignments for OspA N-terminal fragment[27-163]
Deposition date: 2000-06-30 Original release date: 2001-08-09
Authors: Huang, Xiaolin; Nakagawa, Tomoko; Tamura, Atsuo; Link, Karl; Koide, Akiko; Koide, Shohei
Citation: Huang, Xiaolin; Nakagawa, Tomoko; Tamura, Atsuo; Link, Karl; Koide, Akiko; Koide, Shohei. "Formation of the Single-layer Beta-sheet of Borrelia burgdorferi OspA in the Absence of the C-terminal Capping Globular Domain" J. Mol. Biol. 308, 367-375 (2001).
Assembly members:
outer surface protein A, polymer, 141 residues, Formula weight is not available
Natural source: Common Name: Spirochete Taxonomy ID: 139 Superkingdom: Eubacteria Kingdom: Bacteria Genus/species: Borrelia burgdorferi
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
outer surface protein A: GSHMKNSVSVDLPGEMKVLV
SKEKNKDGKYDLIATVDKLE
LKGTSDKNNGSGVLEGVKAD
KSKVKLTISDDLGQTTLEVF
KEDGKTLVSKKVTSKDKSST
EEKFNEKGEVSEKIITRADG
TRLEYTGIKSDGSGKAKEVL
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 138 |
| 13C chemical shifts | 406 |
| 15N chemical shifts | 138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OspA fragment residue 27-163 | 1 |
Entities:
Entity 1, OspA fragment residue 27-163 141 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | LYS | ASN | SER | VAL | SER | VAL | ||||
| 2 | ASP | LEU | PRO | GLY | GLU | MET | LYS | VAL | LEU | VAL | ||||
| 3 | SER | LYS | GLU | LYS | ASN | LYS | ASP | GLY | LYS | TYR | ||||
| 4 | ASP | LEU | ILE | ALA | THR | VAL | ASP | LYS | LEU | GLU | ||||
| 5 | LEU | LYS | GLY | THR | SER | ASP | LYS | ASN | ASN | GLY | ||||
| 6 | SER | GLY | VAL | LEU | GLU | GLY | VAL | LYS | ALA | ASP | ||||
| 7 | LYS | SER | LYS | VAL | LYS | LEU | THR | ILE | SER | ASP | ||||
| 8 | ASP | LEU | GLY | GLN | THR | THR | LEU | GLU | VAL | PHE | ||||
| 9 | LYS | GLU | ASP | GLY | LYS | THR | LEU | VAL | SER | LYS | ||||
| 10 | LYS | VAL | THR | SER | LYS | ASP | LYS | SER | SER | THR | ||||
| 11 | GLU | GLU | LYS | PHE | ASN | GLU | LYS | GLY | GLU | VAL | ||||
| 12 | SER | GLU | LYS | ILE | ILE | THR | ARG | ALA | ASP | GLY | ||||
| 13 | THR | ARG | LEU | GLU | TYR | THR | GLY | ILE | LYS | SER | ||||
| 14 | ASP | GLY | SER | GLY | LYS | ALA | LYS | GLU | VAL | LEU | ||||
| 15 | LYS |
Samples:
sample_1: outer surface protein A, [U-13C; U-15N], 1.5 mM; sodium phosphate 10 mM; sodium chloride 50 mM
set_1: pH: 6.0; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC | sample_1 | not available | set_1 |
| HNCO | sample_1 | not available | set_1 |
| CBCA(CO)NH | sample_1 | not available | set_1 |
| HNCACB | sample_1 | not available | set_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts