BMRB Entry 4848

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4848

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Conformational Changes in the Isolated N-terminal Domain of 5-Enolpyruvylshikimate-3-phosphate Synthase upon Shikimate-3-phosphate Binding PubMed: 11563562

Deposition date: 2000-10-04 Original release date: 2001-11-14

Authors: Stauffer, Melissa; Young, John; Helms, Greg; Evans, Jeremy

Citation: Stauffer, Melissa; Young, John; Helms, Greg; Evans, Jeremy. "Letter to the Editor: Sequential Assignments of the Isolated N-terminal Domain of 5-Enolpyruvylshikimate-3-phosphate Synthase" J. Biomol. NMR 20, 387-388 (2001).

Assembly members:
EPSP synthase, polymer, 240 residues, Formula weight is not available
shikimate-3-phosphate, non-polymer, 254.131 Da.

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Eschericia coli

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
EPSP synthase: MESLTLQPIARVDGTINLPG SKSVSNRALLLAALAHGKTV LTNLLDSDDVRHMLNALTAL GVSYTLSADRTRCEIIGNGG PLHAEGALELFLGNAGTAMR PLAAALCLGSNDIVLTGEPR MKERPIGHLVDALRLGGAKI TYLEQENYPPLRLQGGFTGG NVDVDGSVSSQFLTALLMTA PLAPEDTVIRIKGDLVSKPY IDITLNLMKTFGVEIENQHY QQFVVKGGQSYQSPGTYLVE

Data sets:

assigned_chemical_shifts
- Nterm+S3P_cs

Data type	Count
1H chemical shifts	1546
13C chemical shifts	906
15N chemical shifts	218

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-terminal domain	1
2	shikimate-3-phosphate	2

Entities:

Entity 1, N-terminal domain 240 residues - Formula weight is not available

1	MET	GLU	SER	LEU	THR	LEU	GLN	PRO	ILE	ALA
2	ARG	VAL	ASP	GLY	THR	ILE	ASN	LEU	PRO	GLY
3	SER	LYS	SER	VAL	SER	ASN	ARG	ALA	LEU	LEU
4	LEU	ALA	ALA	LEU	ALA	HIS	GLY	LYS	THR	VAL
5	LEU	THR	ASN	LEU	LEU	ASP	SER	ASP	ASP	VAL
6	ARG	HIS	MET	LEU	ASN	ALA	LEU	THR	ALA	LEU
7	GLY	VAL	SER	TYR	THR	LEU	SER	ALA	ASP	ARG
8	THR	ARG	CYS	GLU	ILE	ILE	GLY	ASN	GLY	GLY
9	PRO	LEU	HIS	ALA	GLU	GLY	ALA	LEU	GLU	LEU
10	PHE	LEU	GLY	ASN	ALA	GLY	THR	ALA	MET	ARG
11	PRO	LEU	ALA	ALA	ALA	LEU	CYS	LEU	GLY	SER
12	ASN	ASP	ILE	VAL	LEU	THR	GLY	GLU	PRO	ARG
13	MET	LYS	GLU	ARG	PRO	ILE	GLY	HIS	LEU	VAL
14	ASP	ALA	LEU	ARG	LEU	GLY	GLY	ALA	LYS	ILE
15	THR	TYR	LEU	GLU	GLN	GLU	ASN	TYR	PRO	PRO
16	LEU	ARG	LEU	GLN	GLY	GLY	PHE	THR	GLY	GLY
17	ASN	VAL	ASP	VAL	ASP	GLY	SER	VAL	SER	SER
18	GLN	PHE	LEU	THR	ALA	LEU	LEU	MET	THR	ALA
19	PRO	LEU	ALA	PRO	GLU	ASP	THR	VAL	ILE	ARG
20	ILE	LYS	GLY	ASP	LEU	VAL	SER	LYS	PRO	TYR
21	ILE	ASP	ILE	THR	LEU	ASN	LEU	MET	LYS	THR
22	PHE	GLY	VAL	GLU	ILE	GLU	ASN	GLN	HIS	TYR
23	GLN	GLN	PHE	VAL	VAL	LYS	GLY	GLY	GLN	SER
24	TYR	GLN	SER	PRO	GLY	THR	TYR	LEU	VAL	GLU

Entity 2, shikimate-3-phosphate 1 residues - C7 H11 O8 P - 254.131 Da.

1

S3P

Samples:

Nterm+S3P: EPSP synthase, [U-15N; U-13C; U70%-2H], 1.0 mM; shikimate-3-phosphate 1.5 mM; Tris buffer 50 mM; DTT 1 mM; NaN3 3 mM; pepstatin uM; leupeptin uM

Ex-cond_1: pH*: 7.8; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Varian Inova 500 MHz

BMRB	4854
PDB	1EPS 1G6S 1G6T 1MI4 1P88 1P89 1Q36 1X8R 1X8T 2AA9 2AAY 2PQ9 2QFQ 2QFS 2QFT 2QFU 3FJX 3FJZ 3FK0 3FK1
DBJ	BAA35643 BAB34414 BAG76491 BAI24350 BAI29802
EMBL	CAA25223 CAP75378 CAQ31436 CAQ97812 CAR02268
GB	AAC32745 AAC72854 AAC73994 AAG55393 AAN42533
PRF	1006223A
REF	NP_286783 NP_309018 NP_415428 NP_706826 NP_752973
SP	A1A9I5 A7ZJZ7 A7ZYL1 B1IW23 B1LJV6

Biological Magnetic Resonance Data Bank