BMRB Entry 4857
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4857
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli
Deposition date: 2000-10-15 Original release date: 2015-09-02
Authors: Ikegami, Takahisa; Okada, Terumasa; Ohki, Izuru; Hirayama, Junya; Mizuno, Takeshi; Shirakawa, Masahiro
Citation: Ikegami, Takahisa; Okada, Terumasa; Ohki, Izuru; Hirayama, Junya; Mizuno, Takeshi; Shirakawa, Masahiro. "Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli" Biochemistry ., .-..
Assembly members:
Histidine-containing phosphotransfer domain of E. coli anaerobic sensor kinase ArcB, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: bacteria Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Histidine-containing phosphotransfer domain of E. coli anaerobic sensor kinase ArcB: TTEENSKSEALLDIPMLEQY
LELVGPKLITDGLAVFEKMM
PGYVSVLESNLTAQDKKGIV
EEGHKIKGAAGSVGLRHLQQ
LGQQIQSPDLPAWEDNVGEW
IEEMKEEWRHDVEVLKAWVA
KATKK
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 513 |
| 15N chemical shifts | 134 |
| 1H chemical shifts | 875 |
| coupling constants | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HPt-ArcB | 1 |
Entities:
Entity 1, HPt-ArcB 125 residues - Formula weight is not available
| 1 | THR | THR | GLU | GLU | ASN | SER | LYS | SER | GLU | ALA | ||||
| 2 | LEU | LEU | ASP | ILE | PRO | MET | LEU | GLU | GLN | TYR | ||||
| 3 | LEU | GLU | LEU | VAL | GLY | PRO | LYS | LEU | ILE | THR | ||||
| 4 | ASP | GLY | LEU | ALA | VAL | PHE | GLU | LYS | MET | MET | ||||
| 5 | PRO | GLY | TYR | VAL | SER | VAL | LEU | GLU | SER | ASN | ||||
| 6 | LEU | THR | ALA | GLN | ASP | LYS | LYS | GLY | ILE | VAL | ||||
| 7 | GLU | GLU | GLY | HIS | LYS | ILE | LYS | GLY | ALA | ALA | ||||
| 8 | GLY | SER | VAL | GLY | LEU | ARG | HIS | LEU | GLN | GLN | ||||
| 9 | LEU | GLY | GLN | GLN | ILE | GLN | SER | PRO | ASP | LEU | ||||
| 10 | PRO | ALA | TRP | GLU | ASP | ASN | VAL | GLY | GLU | TRP | ||||
| 11 | ILE | GLU | GLU | MET | LYS | GLU | GLU | TRP | ARG | HIS | ||||
| 12 | ASP | VAL | GLU | VAL | LEU | LYS | ALA | TRP | VAL | ALA | ||||
| 13 | LYS | ALA | THR | LYS | LYS |
Samples:
sample_1: Histidine-containing phosphotransfer domain of E. coli anaerobic sensor kinase ArcB, [U-99% 13C; U-99% 15N], 1.5 mM
Ex-cond_1: ionic strength: 0.1 M; pH: 6.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| CBCANH | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CA)HA | not available | not available | not available |
| HBHA(CBCACO)NH | not available | not available | not available |
| HBHA(CBCA)NH | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
| (H)C(CO)NH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 15N-edited TOCSY | not available | not available | not available |
| (HB)CB(CGCD)HD | not available | not available | not available |
| (HB)CB(CGCDCE)HE | not available | not available | not available |
Software:
Pipp v4.2.4 - peak picking
NMRPipe v1.7 - data processing
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAB37512 BAE77254 |
| EMBL | CAA37397 |
| GenBank | AAA58012 AAG58344 AAN44715 AAN82410 AAP18529 |
| REF | AP_003753 NP_289784 NP_312116 NP_709008 NP_755836 |
| SWISS-PROT | P0AEC3 P0AEC4 P58363 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts