BMRB Entry 4870
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4870
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit
Deposition date: 2000-10-20 Original release date: 2011-08-10
Authors: Poznanski, Jaroslaw; Zhukow, Igor; Bolewska, Krystyna; Wierzchowski, Kazimierz
Citation: Poznanski, Jaroslaw; Zhukov, Igor; Bolewska, Krystyna; Wierzchowski, Kazimierz. "Letter to the Editor: Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit" J. Biomol. NMR 20, 181-182 (2001).
Assembly members:
region 4 of sigma70 of E. coli RNA polymerase, polymer, 106 residues, 12164 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
region 4 of sigma70 of E. coli RNA polymerase: GSSHHHHHHSSGLVPRGSHM
LELPLDSATTESLRAATHDV
LAGLTAREAKVLRMRFGIDM
NTDYTLEEVGKQFDVTRERI
RQIEAKALRKLRHPSRSEVL
RSFLDD
- assigned_chemical_shifts
- coupling_constants
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
Data type | Count |
1H chemical shifts | 681 |
13C chemical shifts | 435 |
15N chemical shifts | 108 |
coupling constants | 66 |
T1 relaxation values | 94 |
T2 relaxation values | 94 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 4 sigma70 | 1 |
Entities:
Entity 1, 4 sigma70 106 residues - 12164 Da.
1 | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | SER | ||||
2 | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | MET | ||||
3 | LEU | GLU | LEU | PRO | LEU | ASP | SER | ALA | THR | THR | ||||
4 | GLU | SER | LEU | ARG | ALA | ALA | THR | HIS | ASP | VAL | ||||
5 | LEU | ALA | GLY | LEU | THR | ALA | ARG | GLU | ALA | LYS | ||||
6 | VAL | LEU | ARG | MET | ARG | PHE | GLY | ILE | ASP | MET | ||||
7 | ASN | THR | ASP | TYR | THR | LEU | GLU | GLU | VAL | GLY | ||||
8 | LYS | GLN | PHE | ASP | VAL | THR | ARG | GLU | ARG | ILE | ||||
9 | ARG | GLN | ILE | GLU | ALA | LYS | ALA | LEU | ARG | LYS | ||||
10 | LEU | ARG | HIS | PRO | SER | ARG | SER | GLU | VAL | LEU | ||||
11 | ARG | SER | PHE | LEU | ASP | ASP |
Samples:
sample_1: region 4 of sigma70 of E. coli RNA polymerase, [U-13C; U-15N], 3 mM
cond_1: pH: 2.8; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | not available | not available | not available |
2D 1H-13C HSQC | not available | not available | not available |
3D C(CO)NH | not available | not available | not available |
3D CBCA(CO)NH | not available | not available | not available |
3D CBCANH | not available | not available | not available |
3D HNCO | not available | not available | not available |
3D (HCA)CO(CA)NH | not available | not available | not available |
2D J-MODULATED 1H-15N HSQC | not available | not available | not available |
2D H(N)CO | not available | not available | not available |
3D HA(CO)NH | not available | not available | not available |
3D H(CO)NH | not available | not available | not available |
3D 1H-1H-15N NOESY | not available | not available | not available |
Software:
nmrpipe v1.7 - data processing
XEasy v1.3.11 - peak assignment, sequential assignment
NMR spectrometers:
- Varian Unity Plus 500 MHz
Related Database Links:
BMRB | 15930 15936 15975 |
PDB | |
DBJ | BAB37373 BAE77118 BAG66772 BAG78873 BAH65247 |
EMBL | CAD07736 CAL34114 CAP77541 CAQ33404 CAQ90501 |
GB | AAA24601 AAA27242 AAA89147 AAB60181 AAC76103 |
PIR | AB0893 |
REF | NP_289642 NP_311977 NP_417539 NP_457602 NP_462126 |
SP | P00579 P0A2E3 P0A2E4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts