BMRB Entry 4973
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4973
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Title: Sequential Assignment and Secondary Structure of Saratin, an Inhibitor of von Willebrand factor-dependent Platelet Adhesion to Collagen PubMed: 11693575
Deposition date: 2001-03-16 Original release date: 2002-04-04
Authors: Maurer, Till; Bomke, Joerg; Frech, Matthias; Rysoik, Thomas; Kalbitzer, Hans
Citation: Maurer, Till; Bomke, Joerg; Frech, Matthias; Rysoik, Thomas; Kalbitzer, Hans. "Letter to the editor: Sequential Assignment and Secondary Structure of Saratin, an Inhibitor of von Willebrand factor-dependent Platelet Adhesion to Collagen" J. Biomol. NMR 21, 77-78 (2001).
Assembly members:
saratin, polymer, 103 residues, 12068 Da.
Natural source: Common Name: leech Taxonomy ID: 6421 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hirudo medicinalis
Experimental source: Production method: recombinant technology Host organism: Hansenula polymorpha
Entity Sequences (FASTA):
saratin: EEREDCWTFYANRKYTDFDK
SFKKSSDLDECKKTCFKTEY
CYIVFEDTVNKECYYNVVDG
EELDQEKFVVDENFTENYLT
DCEGKDAGNAAGTGDESDEV
DED
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 436 |
15N chemical shifts | 112 |
1H chemical shifts | 667 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | saratin | 1 |
Entities:
Entity 1, saratin 103 residues - 12068 Da.
1 | GLU | GLU | ARG | GLU | ASP | CYS | TRP | THR | PHE | TYR | ||||
2 | ALA | ASN | ARG | LYS | TYR | THR | ASP | PHE | ASP | LYS | ||||
3 | SER | PHE | LYS | LYS | SER | SER | ASP | LEU | ASP | GLU | ||||
4 | CYS | LYS | LYS | THR | CYS | PHE | LYS | THR | GLU | TYR | ||||
5 | CYS | TYR | ILE | VAL | PHE | GLU | ASP | THR | VAL | ASN | ||||
6 | LYS | GLU | CYS | TYR | TYR | ASN | VAL | VAL | ASP | GLY | ||||
7 | GLU | GLU | LEU | ASP | GLN | GLU | LYS | PHE | VAL | VAL | ||||
8 | ASP | GLU | ASN | PHE | THR | GLU | ASN | TYR | LEU | THR | ||||
9 | ASP | CYS | GLU | GLY | LYS | ASP | ALA | GLY | ASN | ALA | ||||
10 | ALA | GLY | THR | GLY | ASP | GLU | SER | ASP | GLU | VAL | ||||
11 | ASP | GLU | ASP |
Samples:
sample_1: saratin 1.5 mM; H2O 100%
sample_2: saratin, [U-15N], 1.5 mM; H2O 100%
sample_3: saratin, [U-5% 13C; U-15N], 1.7 mM; H2O 100%
sample_4: saratin 1.5 mM; D2O 100%
sample_5: saratin, [U-15N], 1.5 mM; D2O 100%
sample_6: saratin, [U-5% 13C; U-15N], 1.7 mM; D2O 100%
Ex-cond_1: ionic strength: 0.067 M; pH: 6.5; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-15N NOESY | not available | not available | Ex-cond_1 |
HNCA | not available | not available | Ex-cond_1 |
HNCO | not available | not available | Ex-cond_1 |
CBCA(CO)NH | not available | not available | Ex-cond_1 |
HCCH-TOCSY | not available | not available | Ex-cond_1 |
NOESY | not available | not available | Ex-cond_1 |
TOCSY | not available | not available | Ex-cond_1 |
Software:
xwinnmr v2.6 - data acqusition, data processing
AURELIA v2.7.10 - automated peak assignment, sequential and sequence specific assignment
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts