BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4973

Title: Sequential Assignment and Secondary Structure of Saratin, an Inhibitor of von Willebrand factor-dependent Platelet Adhesion to Collagen   PubMed: 11693575

Deposition date: 2001-03-16 Original release date: 2002-04-04

Authors: Maurer, Till; Bomke, Joerg; Frech, Matthias; Rysoik, Thomas; Kalbitzer, Hans

Citation: Maurer, Till; Bomke, Joerg; Frech, Matthias; Rysoik, Thomas; Kalbitzer, Hans. "Letter to the editor: Sequential Assignment and Secondary Structure of Saratin, an Inhibitor of von Willebrand factor-dependent Platelet Adhesion to Collagen"  J. Biomol. NMR 21, 77-78 (2001).

Assembly members:
saratin, polymer, 103 residues, 12068 Da.

Natural source:   Common Name: leech   Taxonomy ID: 6421   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hirudo medicinalis

Experimental source:   Production method: recombinant technology   Host organism: Hansenula polymorpha

Entity Sequences (FASTA):
saratin: EEREDCWTFYANRKYTDFDK SFKKSSDLDECKKTCFKTEY CYIVFEDTVNKECYYNVVDG EELDQEKFVVDENFTENYLT DCEGKDAGNAAGTGDESDEV DED

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts436
15N chemical shifts112
1H chemical shifts667

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1saratin1

Entities:

Entity 1, saratin 103 residues - 12068 Da.

1   GLUGLUARGGLUASPCYSTRPTHRPHETYR
2   ALAASNARGLYSTYRTHRASPPHEASPLYS
3   SERPHELYSLYSSERSERASPLEUASPGLU
4   CYSLYSLYSTHRCYSPHELYSTHRGLUTYR
5   CYSTYRILEVALPHEGLUASPTHRVALASN
6   LYSGLUCYSTYRTYRASNVALVALASPGLY
7   GLUGLULEUASPGLNGLULYSPHEVALVAL
8   ASPGLUASNPHETHRGLUASNTYRLEUTHR
9   ASPCYSGLUGLYLYSASPALAGLYASNALA
10   ALAGLYTHRGLYASPGLUSERASPGLUVAL
11   ASPGLUASP

Samples:

sample_1: saratin 1.5 mM; H2O 100%

sample_2: saratin, [U-15N], 1.5 mM; H2O 100%

sample_3: saratin, [U-5% 13C; U-15N], 1.7 mM; H2O 100%

sample_4: saratin 1.5 mM; D2O 100%

sample_5: saratin, [U-15N], 1.5 mM; D2O 100%

sample_6: saratin, [U-5% 13C; U-15N], 1.7 mM; D2O 100%

Ex-cond_1: ionic strength: 0.067 M; pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
NOESYnot availablenot availableEx-cond_1
TOCSYnot availablenot availableEx-cond_1

Software:

xwinnmr v2.6 - data acqusition, data processing

AURELIA v2.7.10 - automated peak assignment, sequential and sequence specific assignment

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 500 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts