BMRB Entry 5009
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5009
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Title: Assignment of 1H,13C and 15N backbone resonances of p13suc1 proteins -- PA90
Deposition date: 2001-05-09 Original release date: 2002-08-22
Authors: Odaert, Benoit; Landrieu, Isabelle; Dijkstra, Klaas; Schuurman-wolters, Gea; Casteels, Peter; Wieruszeski, Jean-Michel; Scheek, Ruud; Lippens, Guy
Citation: Odaert, Benoit; Landrieu, Isabelle; Dijkstra, Klaas; Schuurman-Wolters, Gea; Casteels, Peter; Wieruszeski, Jean-Michel; Scheek, Ruud; Lippens, Guy. "Letter to the Editor: Assignment of the 1H, 13C and 15N resonances and secondary structure of the monomeric p13suc1 protein of Saccharomyces pombe" J. Biomol. NMR 23, 155-156 (2002).
Assembly members:
suc1, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinat technology Host organism: Escherichia coli
Entity Sequences (FASTA):
suc1: MSKSGVPRLLTASERERLEP
FIDQIHYSPRYADDEYEYRH
VMLPKAMLKAIPTDYFNPET
GTLRILQEEEWRGLGITQSL
GWEMYEVHVAEPHILLFKRE
KDYQMKFSQQRGG
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 822 |
13C chemical shifts | 524 |
15N chemical shifts | 120 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | p13 P90A mutant | 1 |
Entities:
Entity 1, p13 P90A mutant 113 residues - Formula weight is not available
1 | MET | SER | LYS | SER | GLY | VAL | PRO | ARG | LEU | LEU | ||||
2 | THR | ALA | SER | GLU | ARG | GLU | ARG | LEU | GLU | PRO | ||||
3 | PHE | ILE | ASP | GLN | ILE | HIS | TYR | SER | PRO | ARG | ||||
4 | TYR | ALA | ASP | ASP | GLU | TYR | GLU | TYR | ARG | HIS | ||||
5 | VAL | MET | LEU | PRO | LYS | ALA | MET | LEU | LYS | ALA | ||||
6 | ILE | PRO | THR | ASP | TYR | PHE | ASN | PRO | GLU | THR | ||||
7 | GLY | THR | LEU | ARG | ILE | LEU | GLN | GLU | GLU | GLU | ||||
8 | TRP | ARG | GLY | LEU | GLY | ILE | THR | GLN | SER | LEU | ||||
9 | GLY | TRP | GLU | MET | TYR | GLU | VAL | HIS | VAL | ALA | ||||
10 | GLU | PRO | HIS | ILE | LEU | LEU | PHE | LYS | ARG | GLU | ||||
11 | LYS | ASP | TYR | GLN | MET | LYS | PHE | SER | GLN | GLN | ||||
12 | ARG | GLY | GLY |
Samples:
sample_1: suc1, [U-15N], 1.0 2.0 mM
sample_2: suc1, [U-13C; U-15N], 1.0 2.0 mM
cond_1: pH: 6.8 na; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
HNCA/HNcaCO | not available | not available | not available |
HNCO/HNcaCO | not available | not available | not available |
CBCANH/CBCAcoNH | not available | not available | not available |
CONCOCAH | not available | not available | not available |
15N NOESY/TOCSY | not available | not available | not available |
13C NOESY/ HCCH TOCSY | not available | not available | not available |
15N HSQC | not available | not available | not available |
13C HSQC aromatic | not available | not available | not available |
Software:
SNARF vv0.8.9 -
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker DMX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts