BMRB Entry 5055

Name: Solution Structure of HI0257, a Bacterial Binding Protein
Published: 2001-10-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5055

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution Structure of HI0257, a Bacterial Binding Protein PubMed: 11551193

Deposition date: 2001-06-13 Original release date: 2001-10-16

Authors: Parsons, L.; Eisenstein, E.; Orban, J.

Citation: Parsons, L.; Eisenstein, E.; Orban, J.. "Solution Structure of HI0257, a Bacterial Ribosome Binding Protein" Biochemistry 40, 10979-10986 (2001).

Assembly members:
HI0257, polymer, 110 residues, 12472 Da.

Natural source: Common Name: Haemophilus influenzae Taxonomy ID: 727 Superkingdom: Eubacteria Kingdom: not available Genus/species: Haemophilus influenzae

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
HI0257: GSHMTLNITSKQMDITPAIR EHLEERLAKLGKWQTQLISP HFVLNKVPNGFSVEASIGTP LGNLLASATSDDMYKAINEV EEKLERQLNKLQHKSESRRA DERLKDSFEN

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	664
13C chemical shifts	397
15N chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HI0257	1

Entities:

Entity 1, HI0257 110 residues - 12472 Da.

1	GLY	SER	HIS	MET	THR	LEU	ASN	ILE	THR	SER
2	LYS	GLN	MET	ASP	ILE	THR	PRO	ALA	ILE	ARG
3	GLU	HIS	LEU	GLU	GLU	ARG	LEU	ALA	LYS	LEU
4	GLY	LYS	TRP	GLN	THR	GLN	LEU	ILE	SER	PRO
5	HIS	PHE	VAL	LEU	ASN	LYS	VAL	PRO	ASN	GLY
6	PHE	SER	VAL	GLU	ALA	SER	ILE	GLY	THR	PRO
7	LEU	GLY	ASN	LEU	LEU	ALA	SER	ALA	THR	SER
8	ASP	ASP	MET	TYR	LYS	ALA	ILE	ASN	GLU	VAL
9	GLU	GLU	LYS	LEU	GLU	ARG	GLN	LEU	ASN	LYS
10	LEU	GLN	HIS	LYS	SER	GLU	SER	ARG	ARG	ALA
11	ASP	GLU	ARG	LEU	LYS	ASP	SER	PHE	GLU	ASN

Samples:

sample_1: HI0257, [U-13C; U-15N], 1.3 mM; NaPO4 50 mM; NaCl 100 mM; dtt 3 mM

sample_cond_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
HNHA	not available	not available	not available
3D HNCACB	not available	not available	not available
3D CBCACONH	not available	not available	not available
3D HNCO	not available	not available	not available
3D HBHACONH	not available	not available	not available
3D 15N TOCSY-HSQC	not available	not available	not available
3D HCCH-TOCSY	not available	not available	not available
2D TOCSY	not available	not available	not available
2D NOESY	not available	not available	not available

Software:

CNS v1.0 - refinement

NMRPipe - processing

Sparky - data analysis

NMR spectrometers:

Bruker DMX 600 MHz
Bruker DMX 500 MHz

PDB	1IMU
EMBL	CBW28505 CBY80445 CBY86679
GB	AAC21923 AAX87316 ABQ97834 ABQ99769 ADO80311
REF	NP_438426 WP_005648863 WP_005653397 WP_005660635 WP_005689430
SP	P71346

Biological Magnetic Resonance Data Bank