BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5107

Title: Assignments of the 1H, 13C, and 15N resonances of the substrate-binding SSD domain from Lon protease   PubMed: 11824761

Deposition date: 2001-08-15 Original release date: 2001-09-12

Authors: Smith, Catherine; Wohnert, Jens; Sauer, Robert; Schwalbe, Harald

Citation: Smith, Catherine; Wohnert, Jens; Sauer, Robert; Schwalbe, Harald. "Letter to the Editor: Assignments of the 1H, 13C, and 15N resonances of the substrate-binding SSD domain from Lon protease"  J. Biomol. NMR 21, 387-388 (2001).

Assembly members:
Lon (La) protease SSD, polymer, 101 residues, 11710 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Lon (La) protease SSD: MRLSGYTEDEKLNIAKRHLL PKQIERNALKKGELTVDDSA IIGIIRYYTREAGVRGLERE ISKLCRKAVKQLLLDKSLKH IEINGDNLHDYLGVQRFDYG R

Data sets:
Data typeCount
1H chemical shifts661
13C chemical shifts421
15N chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Lon SSD1

Entities:

Entity 1, Lon SSD 101 residues - 11710 Da.

1   METARGLEUSERGLYTYRTHRGLUASPGLU
2   LYSLEUASNILEALALYSARGHISLEULEU
3   PROLYSGLNILEGLUARGASNALALEULYS
4   LYSGLYGLULEUTHRVALASPASPSERALA
5   ILEILEGLYILEILEARGTYRTYRTHRARG
6   GLUALAGLYVALARGGLYLEUGLUARGGLU
7   ILESERLYSLEUCYSARGLYSALAVALLYS
8   GLNLEULEULEUASPLYSSERLEULYSHIS
9   ILEGLUILEASNGLYASPASNLEUHISASP
10   TYRLEUGLYVALGLNARGPHEASPTYRGLY
11   ARG

Samples:

sample_1: Lon (La) protease SSD, [U-15N], 3.0 mM

sample_2: Lon (La) protease SSD, [U-15N; U-13C], 3.0 mM

cond_1: pH*: 5.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCC(CO)NHnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available

Software:

NMRpipe v1.8 - processing spectra

nmrView v1.8 - analyzing spectra

NMR spectrometers:

  • Varian UnityInova 750 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAB33916 BAE76219 BAG75989 BAH62055 BAI23813
EMBL CAD08909 CAP74973 CAQ30912 CAQ90073 CAQ97315
GB AAA16837 AAA24079 AAB40195 AAC36871 AAC73542
PIR AE0558
PRF 2004285A
REF NP_286181 NP_308520 NP_414973 NP_455047 NP_459446
SP P0A9M0 P0A9M1 Q32JJ5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts