BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5142

Title: Assignment of 1H, 13C and 15N resonances of Human Lysozyme at 4 C   PubMed: 11883780

Deposition date: 2001-09-06 Original release date: 2002-05-06

Authors: Kumeta, Hiroyuki; Kobashigawa, Yoshihiro; Miura, Kazunori; Nishimiya, Yoshiyuki; Oka, Chitoshi; Nemoto, Nobuaki; Miura, Ai; Nitta, Katsutoshi; Tsuda, Sakae

Citation: Kumeta, Hiroyuki; Kobashigawa, Yoshihiro; Miura, Kazunori; Nishimiya, Yoshiyuki; Oka, Chitoshi; Nemoto, Nobuaki; Miura, Ai; Nitta, Katsutoshi; Tsuda, Sakae. "Letter to the Editor: Assignments of 1H, 13C and 15N Resonances of Human Lysozyme at 4 degrees C"  J. Biomol. NMR 22, 183-184 (2002).

Assembly members:
human lysozyme, polymer, 130 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
human lysozyme: KVFERCELARTLKRLGMDGY RGISLANWMCLAKWESGYNT RATNYNAGDRSTDYGIFQIN SRYWCNDGKTPGAVNACHLS CSALLQDNIADAVACAKRVV RDPQGIRAWVAWRNRCQNRD VRQYVQGCGV

Data sets:
Data typeCount
13C chemical shifts374
15N chemical shifts127
1H chemical shifts708

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human lysozyme1

Entities:

Entity 1, human lysozyme 130 residues - Formula weight is not available

1   LYSVALPHEGLUARGCYSGLULEUALAARG
2   THRLEULYSARGLEUGLYMETASPGLYTYR
3   ARGGLYILESERLEUALAASNTRPMETCYS
4   LEUALALYSTRPGLUSERGLYTYRASNTHR
5   ARGALATHRASNTYRASNALAGLYASPARG
6   SERTHRASPTYRGLYILEPHEGLNILEASN
7   SERARGTYRTRPCYSASNASPGLYLYSTHR
8   PROGLYALAVALASNALACYSHISLEUSER
9   CYSSERALALEULEUGLNASPASNILEALA
10   ASPALAVALALACYSALALYSARGVALVAL
11   ARGASPPROGLNGLYILEARGALATRPVAL
12   ALATRPARGASNARGCYSGLNASNARGASP
13   VALARGGLNTYRVALGLNGLYCYSGLYVAL

Samples:

sample_1: human lysozyme 1.0 mM

HL04-cond_1: pH: 3.8; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
15N-edited NOESYnot availablenot availablenot available
15N-edited TOCSYnot availablenot availablenot available
CBCA(CO)NNHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
13C-edited NOESYnot availablenot availablenot available
1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts