BMRB Entry 5158
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5158
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Title: Conformational and Dynamic Characterization of the Molten Globule state of an Apomyoglobin Mutant with an altered Folding Pathway PubMed: 11724558
Deposition date: 2001-09-25 Original release date: 2002-01-25
Authors: Cavagnero, Silvia; Nishimura, Chiaki; Schwarzinger, Stephan; Dyson, Jane; Wright, Peter
Citation: Cavagnero, Silvia; Nishimura, Chiaki; Schwarzinger, Stephan; Dyson, H.; Wright, Peter. "Conformational and Dynamic Characterization of the Molten Globule State of an Apomyoglobin Mutant with an Altered Folding Pathway" Biochemistry 40, 14459-14467 (2001).
Assembly members:
apomyoglobin, polymer, 153 residues, Formula weight is not available
Natural source: Common Name: sperm whale Taxonomy ID: 9755 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Physeter catodon
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
apomyoglobin: VLSEGEWQLVLHVWAKVEAD
VAGHGQDILIRLFKSHPETL
EKFDRFKHLKTEAEMKASED
LKKHGVTVLTALGAILKKKG
HHEAELKPLAQSHATKHKIP
IKYLEFISEAIIHVLHSRHP
GDFGADAQGAMGKALGLFRK
DIAAKYKELGYQG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 287 |
15N chemical shifts | 137 |
1H chemical shifts | 137 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | apoMb GG mutant | 1 |
Entities:
Entity 1, apoMb GG mutant 153 residues - Formula weight is not available
1 | VAL | LEU | SER | GLU | GLY | GLU | TRP | GLN | LEU | VAL | ||||
2 | LEU | HIS | VAL | TRP | ALA | LYS | VAL | GLU | ALA | ASP | ||||
3 | VAL | ALA | GLY | HIS | GLY | GLN | ASP | ILE | LEU | ILE | ||||
4 | ARG | LEU | PHE | LYS | SER | HIS | PRO | GLU | THR | LEU | ||||
5 | GLU | LYS | PHE | ASP | ARG | PHE | LYS | HIS | LEU | LYS | ||||
6 | THR | GLU | ALA | GLU | MET | LYS | ALA | SER | GLU | ASP | ||||
7 | LEU | LYS | LYS | HIS | GLY | VAL | THR | VAL | LEU | THR | ||||
8 | ALA | LEU | GLY | ALA | ILE | LEU | LYS | LYS | LYS | GLY | ||||
9 | HIS | HIS | GLU | ALA | GLU | LEU | LYS | PRO | LEU | ALA | ||||
10 | GLN | SER | HIS | ALA | THR | LYS | HIS | LYS | ILE | PRO | ||||
11 | ILE | LYS | TYR | LEU | GLU | PHE | ILE | SER | GLU | ALA | ||||
12 | ILE | ILE | HIS | VAL | LEU | HIS | SER | ARG | HIS | PRO | ||||
13 | GLY | ASP | PHE | GLY | ALA | ASP | ALA | GLN | GLY | ALA | ||||
14 | MET | GLY | LYS | ALA | LEU | GLY | LEU | PHE | ARG | LYS | ||||
15 | ASP | ILE | ALA | ALA | LYS | TYR | LYS | GLU | LEU | GLY | ||||
16 | TYR | GLN | GLY |
Samples:
sample_1: apomyoglobin, [U-13C; U-15N], 0.3 mM; ethanol, [U-2H], 10%; H2O 90%
cond1: pH: 4.1 na; temperature: 323 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
HNCA | not available | not available | not available |
HN(CO)CA | not available | not available | not available |
HNCO | not available | not available | not available |
(HCA)CO(CA)NH | not available | not available | not available |
CBCA(CO)NH | not available | not available | not available |
HNCACB | not available | not available | not available |
Software:
NMRPipe -
NMRView -
NMR spectrometers:
- Bruker DMX 750 MHz
Related Database Links:
BMRB | 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4676 4695 823 824 |
PDB | |
DBJ | BAF03579 |
GB | AAA72199 |
PRF | 742482A |
REF | NP_001277651 |
SP | P02185 |
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