BMRB Entry 5228
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5228
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Letter to the Editor: Assignment of a 15 kDa protein complex formed between the p160 coactivator ACTR and the CREB binding protein PubMed: 12018488
Deposition date: 2001-12-10 Original release date: 2002-08-22
Authors: Demarest, Stephen; Chung, John; Dyson, H.; Wright, Peter
Citation: Demarest, Stephen; Chung, John; Dyson, H.; Wright, Peter. "Letter to the Editor: Assignment of a 15 kDa protein complex formed between the p160 coactivator ACTR and the CREB binding protein" J. Biomol. NMR 22, 377-378 (2002).
Assembly members:
ACTR, polymer, 71 residues, Formula weight is not available
CREB binding protein, polymer, 59 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ACTR: GTQNRPLLRNSLDDLVGPPS
NLEGQSDERALLDQLHTLLS
NTDATGLEEIDRALGIPELV
NQGQALEPKQD
CREB binding protein: PNRSISPSALQDLLRTLKSP
SSPQQQQQVLNILKSNPQLM
AAFIKQRTAKYVANQPGMQ
| Data type | Count |
| 1H chemical shifts | 743 |
| 13C chemical shifts | 480 |
| 15N chemical shifts | 119 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ACTR | 1 |
| 2 | CREB binding protein | 2 |
Entities:
Entity 1, ACTR 71 residues - Formula weight is not available
| 1 | GLY | THR | GLN | ASN | ARG | PRO | LEU | LEU | ARG | ASN | ||||
| 2 | SER | LEU | ASP | ASP | LEU | VAL | GLY | PRO | PRO | SER | ||||
| 3 | ASN | LEU | GLU | GLY | GLN | SER | ASP | GLU | ARG | ALA | ||||
| 4 | LEU | LEU | ASP | GLN | LEU | HIS | THR | LEU | LEU | SER | ||||
| 5 | ASN | THR | ASP | ALA | THR | GLY | LEU | GLU | GLU | ILE | ||||
| 6 | ASP | ARG | ALA | LEU | GLY | ILE | PRO | GLU | LEU | VAL | ||||
| 7 | ASN | GLN | GLY | GLN | ALA | LEU | GLU | PRO | LYS | GLN | ||||
| 8 | ASP |
Entity 2, CREB binding protein 59 residues - Formula weight is not available
| 1 | PRO | ASN | ARG | SER | ILE | SER | PRO | SER | ALA | LEU | ||||
| 2 | GLN | ASP | LEU | LEU | ARG | THR | LEU | LYS | SER | PRO | ||||
| 3 | SER | SER | PRO | GLN | GLN | GLN | GLN | GLN | VAL | LEU | ||||
| 4 | ASN | ILE | LEU | LYS | SER | ASN | PRO | GLN | LEU | MET | ||||
| 5 | ALA | ALA | PHE | ILE | LYS | GLN | ARG | THR | ALA | LYS | ||||
| 6 | TYR | VAL | ALA | ASN | GLN | PRO | GLY | MET | GLN |
Samples:
sample_1: ACTR, [U-95% 13C; U-90% 15N], 1.0 – 5.0 mM; CREB binding protein1.0 – 5.0 mM; TrisHCl 10 mM; NaCl 50 mM
sample_2: ACTR, [U-90% 15N], 1.0 – 5.0 mM; CREB binding protein1.0 – 5.0 mM; TrisHCl 10 mM; NaCl 50 mM
sample_3: ACTR1.0 – 5.0 mM; CREB binding protein, [U-95% 13C; U-90% 15N], 1.0 – 5.0 mM; TrisHCl 10 mM; NaCl 50 mM
sample_4: ACTR1.0 – 5.0 mM; CREB binding protein, [U-90% 15N], 1.0 – 5.0 mM; TrisHCl 10 mM; NaCl 50 mM
Native_cond: pH: 6.6; temperature: 304 K; ionic strength: 0.06 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N HSQC NOESY | not available | not available | not available |
| 3D 1H-13C HSQC NOESY | not available | not available | not available |
| 3D 12C-filtered/1H-13C-edited NOESY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HBHACONH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| 2D CGCO | not available | not available | not available |
| 2D CGCN | not available | not available | not available |
| 3D HACACB-COSY | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D HNHB | not available | not available | not available |
| 3D HCCH-TOCSY | not available | not available | not available |
| 3D CCH-TOCSY | not available | not available | not available |
| 3D CCH-COSY | not available | not available | not available |
| 3D 1H-15N HSQC TOCSY | not available | not available | not available |
Software:
NMRVIEW v3.0 - Processing
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz
- Bruker DMX 600 MHz
- Bruker AMX 600 MHz
- Bruker DMX 750 MHz
- Bruker DRX 800 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts