BMRB Entry 5267
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5267
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Title: 1H, 13C, 15N NMR sequencespecific resonance assignment of a Clostridium thermocellum type II cohesin module PubMed: 12061722
Deposition date: 2002-01-28 Original release date: 2002-08-23
Authors: Smith, Steven; Beguin, Pierre; Alzari, Pedro; Gehring, Kalle
Citation: Smith, Steven; Beguin, Pierre; Alzari, Pedro; Gehring, Kalle. "Letter to the Editor: 1H, 13C, 15N NMR sequence-specific resonance assignment of a Clostridium thermocellum type II cohesin module " J. Biomol. NMR 23, 73-74 (2002).
Assembly members:
SdbA type II cohesin, polymer, 187 residues, Formula weight is not available
Natural source: Common Name: Clostridium thermocellum Taxonomy ID: 1515 Superkingdom: Eubacteria Kingdom: not available Genus/species: Clostridium thermocellum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SdbA type II cohesin: MRGSHHHHHHTDLRADKASS
IELKFDRNKGEVGDILIGTV
RINNIKNFAGFQVNIVYDPK
VLMAVDPETGKEFTSSTFPP
GRTVLKNNAYGPIQIADNDP
EKGILNFALAYSYIAGYKET
GVAEESGIIAKIGFKILQKK
STAVKFQDTLSMPGAISGTQ
LFDWDGEVITGYEVIQPDVL
SLGDEPF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 966 |
| 13C chemical shifts | 650 |
| 15N chemical shifts | 162 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SdbA cohesin module | 1 |
Entities:
Entity 1, SdbA cohesin module 187 residues - Formula weight is not available
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | THR | ASP | LEU | ARG | ALA | ASP | LYS | ALA | SER | SER | ||||
| 3 | ILE | GLU | LEU | LYS | PHE | ASP | ARG | ASN | LYS | GLY | ||||
| 4 | GLU | VAL | GLY | ASP | ILE | LEU | ILE | GLY | THR | VAL | ||||
| 5 | ARG | ILE | ASN | ASN | ILE | LYS | ASN | PHE | ALA | GLY | ||||
| 6 | PHE | GLN | VAL | ASN | ILE | VAL | TYR | ASP | PRO | LYS | ||||
| 7 | VAL | LEU | MET | ALA | VAL | ASP | PRO | GLU | THR | GLY | ||||
| 8 | LYS | GLU | PHE | THR | SER | SER | THR | PHE | PRO | PRO | ||||
| 9 | GLY | ARG | THR | VAL | LEU | LYS | ASN | ASN | ALA | TYR | ||||
| 10 | GLY | PRO | ILE | GLN | ILE | ALA | ASP | ASN | ASP | PRO | ||||
| 11 | GLU | LYS | GLY | ILE | LEU | ASN | PHE | ALA | LEU | ALA | ||||
| 12 | TYR | SER | TYR | ILE | ALA | GLY | TYR | LYS | GLU | THR | ||||
| 13 | GLY | VAL | ALA | GLU | GLU | SER | GLY | ILE | ILE | ALA | ||||
| 14 | LYS | ILE | GLY | PHE | LYS | ILE | LEU | GLN | LYS | LYS | ||||
| 15 | SER | THR | ALA | VAL | LYS | PHE | GLN | ASP | THR | LEU | ||||
| 16 | SER | MET | PRO | GLY | ALA | ILE | SER | GLY | THR | GLN | ||||
| 17 | LEU | PHE | ASP | TRP | ASP | GLY | GLU | VAL | ILE | THR | ||||
| 18 | GLY | TYR | GLU | VAL | ILE | GLN | PRO | ASP | VAL | LEU | ||||
| 19 | SER | LEU | GLY | ASP | GLU | PRO | PHE |
Samples:
sample_1: SdbA type II cohesin, [U-95% 13C; U-90% 15N], 1.2 mM
sample_2: SdbA type II cohesin, [U-90% 15N], 1.2 mM
Excond_1: pH: 6.9 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H15N HSQC | not available | not available | not available |
| 1H 13C HSQC | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 15N NOESY HSQC | not available | not available | not available |
| CC(CO)NH TOCSY | not available | not available | not available |
| HCC(CO)NH TOCSY | not available | not available | not available |
| (H)CCH COSY | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
Software:
XEASY - peak assignment
NMR spectrometers:
- Bruker AVANCE 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| EMBL | CDG35978 |
| GB | AAB07763 ABN52539 ADU74018 EEU01806 EFB39640 |
| REF | WP_003516982 WP_004463692 YP_001037732 YP_005687469 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts