BMRB Entry 5368

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for hARF1
Published: 2005-11-14

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5368

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for hARF1 PubMed: 12238602

Deposition date: 2002-05-09 Original release date: 2005-11-14

Authors: Amor, Juan Carlos; Seidel, Ronald; Tian, Fang; Kahn, Richard; Prestegard, James

Citation: Amor, Juan Carlos; Seidel, Ronald; Tian, Fang; Kahn, Richard; Prestegard, James. "Letter to the Editor: 1H, 15N, and 13C Assignments of Full Length Human ADP Ribosylation Factor 1 (ARF1) using Triple Resonance Connectivities and Dipolar Couplings" J. Biomol. NMR 23, 253-254 (2002).

Assembly members:
ADP Ribosylation Factor 1, polymer, 180 residues, 20562 Da.
GUANOSINE-5'-DIPHOSPHATE, non-polymer, 443.201 Da.
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
ADP Ribosylation Factor 1: GNIFANLFKGLFGKKEMRIL MVGLDAAGKTTILYKLKLGE IVTTIPTIGFNVETVEYKNI SFTVWDVGGQDKIRPLWRHY FQNTQGLIFVVDSNDRERVN EAREELMRMLAEDELRDAVL LVFANKQDLPNAMNAAEITD KLGLHSLRHRNWYIQATCAT SGDGLYEGLDWLSNQLRNQK

Data sets:

assigned_chemical_shifts
- ARF1_Chemical_Shifts

Data type	Count
1H chemical shifts	164
13C chemical shifts	474
15N chemical shifts	164

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hARF1 monomer	1
2	GUANOSINE-5'-DIPHOSPHATE	2
3	MG 2+	3

Entities:

Entity 1, hARF1 monomer 180 residues - 20562 Da.

1	GLY	ASN	ILE	PHE	ALA	ASN	LEU	PHE	LYS	GLY
2	LEU	PHE	GLY	LYS	LYS	GLU	MET	ARG	ILE	LEU
3	MET	VAL	GLY	LEU	ASP	ALA	ALA	GLY	LYS	THR
4	THR	ILE	LEU	TYR	LYS	LEU	LYS	LEU	GLY	GLU
5	ILE	VAL	THR	THR	ILE	PRO	THR	ILE	GLY	PHE
6	ASN	VAL	GLU	THR	VAL	GLU	TYR	LYS	ASN	ILE
7	SER	PHE	THR	VAL	TRP	ASP	VAL	GLY	GLY	GLN
8	ASP	LYS	ILE	ARG	PRO	LEU	TRP	ARG	HIS	TYR
9	PHE	GLN	ASN	THR	GLN	GLY	LEU	ILE	PHE	VAL
10	VAL	ASP	SER	ASN	ASP	ARG	GLU	ARG	VAL	ASN
11	GLU	ALA	ARG	GLU	GLU	LEU	MET	ARG	MET	LEU
12	ALA	GLU	ASP	GLU	LEU	ARG	ASP	ALA	VAL	LEU
13	LEU	VAL	PHE	ALA	ASN	LYS	GLN	ASP	LEU	PRO
14	ASN	ALA	MET	ASN	ALA	ALA	GLU	ILE	THR	ASP
15	LYS	LEU	GLY	LEU	HIS	SER	LEU	ARG	HIS	ARG
16	ASN	TRP	TYR	ILE	GLN	ALA	THR	CYS	ALA	THR
17	SER	GLY	ASP	GLY	LEU	TYR	GLU	GLY	LEU	ASP
18	TRP	LEU	SER	ASN	GLN	LEU	ARG	ASN	GLN	LYS

Entity 2, GUANOSINE-5'-DIPHOSPHATE 1 residues - C10 H15 N5 O11 P2 - 443.201 Da.

1

GDP

Entity 3, MG 2+ - Mg - 24.305 Da.

1

MG

Samples:

sample_1: ADP Ribosylation Factor 1 0.5 mM; GUANOSINE-5'-DIPHOSPHATE mM; magnesium mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCO	not available	not available	not available
CBCACONH	not available	not available	not available
HNCACB	not available	not available	not available

Software:

FELIX v2000 -

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1HUR 1J2J 1O3Y 1R8Q 1R8S 1RE0 1RRF 1RRG 1S9D 1U81 2J59 3O47 4C0A 4HMY
DBJ	BAA13490 BAE01583 BAE28566 BAE28685 BAE29300
EMBL	CAG03028 CAG31143 CAJ81862 CAL38139 CAL38339
GB	AAA30361 AAA35511 AAA35512 AAA35552 AAA40685
PRF	2004472A
REF	NP_001001905 NP_001006352 NP_001019397 NP_001019398 NP_001019399
SP	P51643 P84077 P84078 P84079 P84080
TPG	DAA28323 DAA29912 DAA31858

Biological Magnetic Resonance Data Bank