BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5504

Title: 1H, 13C and 15N chemical shift assignments of the SH2 domain of the Csk homologous kinase

Deposition date: 2002-08-20 Original release date: 2002-12-23

Authors: Mulhern, Terrence; To, Catherine; Cheng, Heung-Chin

Citation: Mulhern, Terrence; To, Catherine; Cheng, Heung-Chin. "Letter to the Editor: 1H, 13C and 15N chemical shift assignments of the SH2 domain of the Csk homologous kinase"  J. Biomol. NMR 24, 363-364 (2002).

Assembly members:
Csk homologous kinase Src homology-2 domain, polymer, 123 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Csk homologous kinase Src homology-2 domain: GPLGSALSTDPKLSLMPWFH GKISGQEAIQQLQPPEDGLF LVRESARHPGDYVLCVSFGR DVIHYRVLHRDGHLTIDEAV CFCNLMDMVEHYTRDKGAIC TKLVKPKRKQGAKSAEEELA KAG

Data sets:
Data typeCount
13C chemical shifts352
15N chemical shifts122
1H chemical shifts868
coupling constants102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CHK SH2 subunit 11

Entities:

Entity 1, CHK SH2 subunit 1 123 residues - Formula weight is not available

1   GLYPROLEUGLYSERALALEUSERTHRASP
2   PROLYSLEUSERLEUMETPROTRPPHEHIS
3   GLYLYSILESERGLYGLNGLUALAILEGLN
4   GLNLEUGLNPROPROGLUASPGLYLEUPHE
5   LEUVALARGGLUSERALAARGHISPROGLY
6   ASPTYRVALLEUCYSVALSERPHEGLYARG
7   ASPVALILEHISTYRARGVALLEUHISARG
8   ASPGLYHISLEUTHRILEASPGLUALAVAL
9   CYSPHECYSASNLEUMETASPMETVALGLU
10   HISTYRTHRARGASPLYSGLYALAILECYS
11   THRLYSLEUVALLYSPROLYSARGLYSGLN
12   GLYALALYSSERALAGLUGLUGLULEUALA
13   LYSALAGLY

Samples:

sample_1: Csk homologous kinase Src homology-2 domain, [U-99% 13C; U-99% 15N], 0.5 mM

sample_2: Csk homologous kinase Src homology-2 domain, [U-99% 15N], 0.2 mM

sample_3: Csk homologous kinase Src homology-2 domain 0.2 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H DQF-COSYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 500 MHz

Related Database Links:

DBJ BAA08199 BAB28926
EMBL CAA58806
GB AAA18829 AAA64524 AAB33566 AAB59677 AAH87726
REF NP_001272782 NP_001272783 NP_001272784 NP_034898 NP_068631
SP P41242 P41243

Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone or all simulated shifts