BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5595

Title: Letter to the Editor: 1H, 13C, 15N backbone resonance assignments of apo-NosL, a novel 18.5 kDa copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes   PubMed: 15014237

Deposition date: 2002-11-21 Original release date: 2004-03-15

Authors: Taubner, Lara; McGuirl, Michele; Dooley, David; Copie, Valerie

Citation: Taubner, Lara; McGuirl, Michele; Dooley, David; Copie, Valerie. "Letter to the Editor: 1H, 13C, 15N backbone and sidechain resonance assignments of apo-NosL, a novel copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes"  J. Biomol. NMR 29, 211-212 (2004).

Assembly members:
NosL, polymer, 175 residues, 18538 Da.

Natural source:   Common Name: Denitrifying bacteria   Taxonomy ID: 223   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Achromobacter cycloclastes

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NosL: MDKEDVAQSIVPQDMTPETL GHYCQMNLLEHPGPKAQIFL EGSPAPLFFSQVRDAIAYAR GPEQIAPILVIYVNDMGAAG ATWDQPGDGNWIAADKAFYV VGSARRGGMGAPEAVPFSSR DEAAAFVLAEGGQVLALADI TDAMVLTPVETGSEPRADDE DYLGRLRALPHPAGG

Data sets:
Data typeCount
1H chemical shifts846
13C chemical shifts596
15N chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NosL monomer1

Entities:

Entity 1, NosL monomer 175 residues - 18538 Da.

1   METASPLYSGLUASPVALALAGLNSERILE
2   VALPROGLNASPMETTHRPROGLUTHRLEU
3   GLYHISTYRCYSGLNMETASNLEULEUGLU
4   HISPROGLYPROLYSALAGLNILEPHELEU
5   GLUGLYSERPROALAPROLEUPHEPHESER
6   GLNVALARGASPALAILEALATYRALAARG
7   GLYPROGLUGLNILEALAPROILELEUVAL
8   ILETYRVALASNASPMETGLYALAALAGLY
9   ALATHRTRPASPGLNPROGLYASPGLYASN
10   TRPILEALAALAASPLYSALAPHETYRVAL
11   VALGLYSERALAARGARGGLYGLYMETGLY
12   ALAPROGLUALAVALPROPHESERSERARG
13   ASPGLUALAALAALAPHEVALLEUALAGLU
14   GLYGLYGLNVALLEUALALEUALAASPILE
15   THRASPALAMETVALLEUTHRPROVALGLU
16   THRGLYSERGLUPROARGALAASPASPGLU
17   ASPTYRLEUGLYARGLEUARGALALEUPRO
18   HISPROALAGLYGLY

Samples:

15N_13C_sample: NosL, [U-15N; U-13C], 1.0 mM; DTT, [U-2D], 1.0 mM; EDTA 1.0 mM; D2O 10%; sodium phosphate 100 mM

15N_13C_NosL_cond: pH*: 6.5; temperature: 303 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CCONHnot availablenot availablenot available
HCCONHnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
15N-TOCSYnot availablenot availablenot available
15N-NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

NMRPipe - processing, peak picking, peak tables

PIPP - processing, peak picking, peak tables

XWINNMR v3.1 - processing

NMR spectrometers:

  • BRUKER DRX 600 MHz

Related Database Links:

PDB
EMBL CAA75429
GB AAD09161 KDP72987
REF WP_029742711 WP_034799000

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts