BMRB Entry 5772
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5772
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C and 15N chemical shifts of CBM13 from Streptomyces lividans PubMed: 11914071
Deposition date: 2003-03-14 Original release date: 2003-05-22
Authors: Schaerpf, Manuela; McIntosh, Lawrence
Citation: Scharpf, Manuela; Connelly, G.; Lee, G.; Boraston, A.; Warren, R.; McIntosh, Lawrence. "Site-specific Characterization of the Association of Xylooligosaccharides with the CBM13 Lectin-like Xylan Binding Domain from Streptomyces lividans Xylanase 10A by NMR Spectroscopy " Biochemistry 41, 4255-4263 (2002).
Assembly members:
family 13 carbohydrate binding modul, polymer, 142 residues, 15035.3 Da.
Natural source: Common Name: Streptomyces lividans Taxonomy ID: 1916 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces lividans
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
family 13 carbohydrate binding modul: HHHHHHIEGRASEPPADGGQ
IKGVGSGRCLDVPDASTSDG
TQLQLWDCHSGTNQQWAATD
AGELRVYGDKCLDAAGTSNG
SKVQIYSCWGGDNQKWRLNS
DGSVVGVQSGLCLDAVGNGT
ANGTLIQLYTCSNGSNQRWT
RT
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 537 |
13C chemical shifts | 481 |
15N chemical shifts | 149 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | CBM13 | 1 |
Entities:
Entity 1, CBM13 142 residues - 15035.3 Da.
1 | HIS | HIS | HIS | HIS | HIS | HIS | ILE | GLU | GLY | ARG | ||||
2 | ALA | SER | GLU | PRO | PRO | ALA | ASP | GLY | GLY | GLN | ||||
3 | ILE | LYS | GLY | VAL | GLY | SER | GLY | ARG | CYS | LEU | ||||
4 | ASP | VAL | PRO | ASP | ALA | SER | THR | SER | ASP | GLY | ||||
5 | THR | GLN | LEU | GLN | LEU | TRP | ASP | CYS | HIS | SER | ||||
6 | GLY | THR | ASN | GLN | GLN | TRP | ALA | ALA | THR | ASP | ||||
7 | ALA | GLY | GLU | LEU | ARG | VAL | TYR | GLY | ASP | LYS | ||||
8 | CYS | LEU | ASP | ALA | ALA | GLY | THR | SER | ASN | GLY | ||||
9 | SER | LYS | VAL | GLN | ILE | TYR | SER | CYS | TRP | GLY | ||||
10 | GLY | ASP | ASN | GLN | LYS | TRP | ARG | LEU | ASN | SER | ||||
11 | ASP | GLY | SER | VAL | VAL | GLY | VAL | GLN | SER | GLY | ||||
12 | LEU | CYS | LEU | ASP | ALA | VAL | GLY | ASN | GLY | THR | ||||
13 | ALA | ASN | GLY | THR | LEU | ILE | GLN | LEU | TYR | THR | ||||
14 | CYS | SER | ASN | GLY | SER | ASN | GLN | ARG | TRP | THR | ||||
15 | ARG | THR |
Samples:
sample_1: family 13 carbohydrate binding modul, [U-13C; U-15N], 1 mM; potassium phosphate 50 mM; potassium chloride 50 mM
SlxA_CBM13_conditions: pH: 6.1 na; temperature: 303 K; ionic strength: 0.1 M
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-15N-HSQC | not available | not available | not available |
HNCACB | not available | not available | not available |
CBCACONH | not available | not available | not available |
CC-TOCSY | not available | not available | not available |
HCC-TOCSY | not available | not available | not available |
HNCO | not available | not available | not available |
HNHA | not available | not available | not available |
HNHB | not available | not available | not available |
HMQC-NH2 | not available | not available | not available |
Software:
FELIX v95 and 2000 -
NMR spectrometers:
- VARIAN UNITY 500 MHz
Related Database Links:
PDB | |
EMBL | CAD55241 |
GB | AAC26525 AIJ12797 EFD66158 EOY50917 |
REF | NP_733679 WP_003973090 WP_011030540 |
SP | P26514 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts