BMRB Entry 5792
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5792
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Title: Assignment of the 1H, 15N and 13C resonances of E. coli frataxin orthologue, CyaY PubMed: 14512742
Deposition date: 2003-05-06 Original release date: 2004-06-24
Authors: Nair, Margie; Adinolfi, Salvatore; Kelly, Geoff; Frenkiel, Thomas; Pastore, Annalisa
Citation: Nair, Margie; Adinolfi, Salvatore; Kelly, Geoff; Frenkiel, Thomas; Pastore, Annalisa. "Letter to the Editor: NMR assignment of the 1H, 15N and 13C resonances of E. coli frataxin orthologue, CyaY " J. Biomol. NMR 27, 403-404 (2003).
Assembly members:
Escherichia coli CyaY, polymer, 109 residues, 12415 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Escherichia coli CyaY: GAMGNDSEFHRLADQLWLTI
EERLDDWDGDSDIDCEINGG
VLTITFENGSKIIINRQEPL
HQVWLATKQGGYHFDLKGDE
WICDRSGETFWDLLEQAATQ
QAGETVSFR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 434 |
| 15N chemical shifts | 116 |
| 1H chemical shifts | 691 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CyaY monomer | 1 |
Entities:
Entity 1, CyaY monomer 109 residues - 12415 Da.
| 1 | GLY | ALA | MET | GLY | ASN | ASP | SER | GLU | PHE | HIS | ||||
| 2 | ARG | LEU | ALA | ASP | GLN | LEU | TRP | LEU | THR | ILE | ||||
| 3 | GLU | GLU | ARG | LEU | ASP | ASP | TRP | ASP | GLY | ASP | ||||
| 4 | SER | ASP | ILE | ASP | CYS | GLU | ILE | ASN | GLY | GLY | ||||
| 5 | VAL | LEU | THR | ILE | THR | PHE | GLU | ASN | GLY | SER | ||||
| 6 | LYS | ILE | ILE | ILE | ASN | ARG | GLN | GLU | PRO | LEU | ||||
| 7 | HIS | GLN | VAL | TRP | LEU | ALA | THR | LYS | GLN | GLY | ||||
| 8 | GLY | TYR | HIS | PHE | ASP | LEU | LYS | GLY | ASP | GLU | ||||
| 9 | TRP | ILE | CYS | ASP | ARG | SER | GLY | GLU | THR | PHE | ||||
| 10 | TRP | ASP | LEU | LEU | GLU | GLN | ALA | ALA | THR | GLN | ||||
| 11 | GLN | ALA | GLY | GLU | THR | VAL | SER | PHE | ARG |
Samples:
15N_sample: Escherichia coli CyaY, [U-15N], 1 – 2 mM
13C_sample: Escherichia coli CyaY, [U-13C; U-15N], 1 – 2 mM
condition_set-1: pH*: 7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H-15N NOESY-HSQC | not available | not available | condition_set-1 |
| 1H-1H-13C TOCSY-HSQC | not available | not available | condition_set-1 |
| 3D HNHA | not available | not available | condition_set-1 |
| 3D HNHB | not available | not available | condition_set-1 |
| 3D HNCA | not available | not available | condition_set-1 |
| 3D HN(CO)CA | not available | not available | condition_set-1 |
| 3D HNC0 | not available | not available | condition_set-1 |
| 3D CBCA(CO)NH | not available | not available | condition_set-1 |
| 1H-1H-13C HCCH-TOCSY | not available | not available | condition_set-1 |
| 1H-1H-13C NOESY-HSQC | not available | not available | condition_set-1 |
| HCC(CO)NH | not available | not available | condition_set-1 |
Software:
XEASY v1.2 - peak assignment
NMRPipe - spectral processing
NMR spectrometers:
- Varian UnityPlus 500 MHz
- Varian Unity 600 MHz
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Related Database Links:
| BMRB | 15237 15244 |
| PDB | |
| DBJ | BAB38160 BAE77494 BAG79612 BAI27941 BAI33064 |
| EMBL | CAA47281 CAP78250 CAQ34148 CAQ91159 CAR00776 |
| GB | AAA67603 AAC76810 AAG58999 AAN45316 AAN83159 |
| REF | NP_290435 NP_312764 NP_418251 NP_709609 NP_756585 |
| SP | A1AHX3 A7ZU09 A8A6R1 B1IW97 B1LLX7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts