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BMRB Entry 5822

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5822
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Title: 1H, 15N and 13C assignments of single-stranded DNA binding domains from the 70 kDa subunit of Human Replication Protein A   PubMed: 14755166

Deposition date: 2003-06-06 Original release date: 2004-02-11

Authors: Bhattacharya, Shibani; Arunkumar, Alphonse; Sullivan, Shannon; Botuyan, Maria-Victoria; Arrowsmith, Cheryl; Chazin, Walter

Citation: Bhattacharya, Shibani; Arunkumar, Alphonse; Sullivan, Shannon; Botuyan, Maria-Victoria; Arrowsmith, Cheryl; Chazin, Walter. "Letter to the Editor: 1H, 13C and 15N assignments of single-stranded DNA binding domains from the 70 kDa subunit of Human Replication Protein A "  J. Biomol. NMR 28, 195-196 (2004).

Assembly members:
single chain biopolymer, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: 9606   Taxonomy ID: Human   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
single chain biopolymer: QFDFTGIDDLENKSKDSLVD IIGICKSYEDATKITVRSNN REVAKRNIYLMDTSGKVVTA TLWGEDADKFDGSRQPVLAI KGARVSDFGGRSLSVLSSST IIANPDIPEAYKLRGWFDAE GQAL

Data sets:
Data typeCount
1H chemical shifts845
13C chemical shifts532
15N chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RPA70(299-422)1

Entities:

Entity 1, RPA70(299-422) 124 residues - Formula weight is not available

1   GLNPHEASPPHETHRGLYILEASPASPLEU
2   GLUASNLYSSERLYSASPSERLEUVALASP
3   ILEILEGLYILECYSLYSSERTYRGLUASP
4   ALATHRLYSILETHRVALARGSERASNASN
5   ARGGLUVALALALYSARGASNILETYRLEU
6   METASPTHRSERGLYLYSVALVALTHRALA
7   THRLEUTRPGLYGLUASPALAASPLYSPHE
8   ASPGLYSERARGGLNPROVALLEUALAILE
9   LYSGLYALAARGVALSERASPPHEGLYGLY
10   ARGSERLEUSERVALLEUSERSERSERTHR
11   ILEILEALAASNPROASPILEPROGLUALA
12   TYRLYSLEUARGGLYTRPPHEASPALAGLU
13   GLYGLNALALEU

Samples:

Sample_1: single chain biopolymer, [U-15N; U-13C], 0.5 – 1.0 mM; tris-d11 20 mM; Potassium Chloride 50 mM; Magnesium Chloride 10 mM; DTT 2 mM; Sodium Azide 0.01%; H2O 90%; D2O 10%

sample_cond_1: pH: 7.2; temperature: 298 K; ionic strength: 0.080 M

Experiments:

NameSampleSample stateSample conditions
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
H(CCO)NH-TOCSYnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available

Software:

FELIX v2000 -

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16754 5823
PDB
DBJ BAD92969 BAE02429 BAF82393 BAG10883
EMBL CAH92206
GB AAA36584 AAH18126 AAS94324 ADR83314 AIC58576
REF NP_001126290 NP_001270333 NP_002936 XP_002800290 XP_003277864
SP P27694 Q5R7Q4

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