BMRB Entry 5826
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5826
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Title: Nickel-binding property of Bacillus pasteurii UreE and the role of the C-terminal tail PubMed: 14769802
Deposition date: 2003-06-09 Original release date: 2004-04-06
Authors: Lee, Yeon-Hee; Won, Hyung-Sik; Lee, Bong-Jin
Citation: Won, Hyung-Sik; Lee, Yeon-Hee; Kim, J.; Shin, I.; Lee, M.; Lee, Bong-Jin. "Structural characterization of the nickel-binding properties of bacillus pasteurii UreE in solution" J. Biol. Chem. ., .-. (2004).
Assembly members:
Bacillus pasteurii UreE, polymer, 147 residues, Formula weight is not available
Natural source: Common Name: Bacillus pasteurii Taxonomy ID: 1474 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus pasteurii
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Bacillus pasteurii UreE: MLITKIVGHIDDYESSDKKV
DWLEVEWEDLNKRILRKETE
NGTDIAIKLENSGTLRYGDV
LYESDDTLIAIRTKLEKVYV
IKPQTMQEMGKMAFEIGNRH
TMCIIEDDEILVRYDKTLEK
LIDEVGVSYEQSERRFKEPF
KYRGHQH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 378 |
| 15N chemical shifts | 125 |
| 1H chemical shifts | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BpUreE subunit 1 | 1 |
| 2 | BpUreE subunit 2 | 1 |
| 3 | BpUreE subunit 3 | 1 |
| 4 | BpUreE subunit 4 | 1 |
Entities:
Entity 1, BpUreE subunit 1 147 residues - Formula weight is not available
| 1 | MET | LEU | ILE | THR | LYS | ILE | VAL | GLY | HIS | ILE | ||||
| 2 | ASP | ASP | TYR | GLU | SER | SER | ASP | LYS | LYS | VAL | ||||
| 3 | ASP | TRP | LEU | GLU | VAL | GLU | TRP | GLU | ASP | LEU | ||||
| 4 | ASN | LYS | ARG | ILE | LEU | ARG | LYS | GLU | THR | GLU | ||||
| 5 | ASN | GLY | THR | ASP | ILE | ALA | ILE | LYS | LEU | GLU | ||||
| 6 | ASN | SER | GLY | THR | LEU | ARG | TYR | GLY | ASP | VAL | ||||
| 7 | LEU | TYR | GLU | SER | ASP | ASP | THR | LEU | ILE | ALA | ||||
| 8 | ILE | ARG | THR | LYS | LEU | GLU | LYS | VAL | TYR | VAL | ||||
| 9 | ILE | LYS | PRO | GLN | THR | MET | GLN | GLU | MET | GLY | ||||
| 10 | LYS | MET | ALA | PHE | GLU | ILE | GLY | ASN | ARG | HIS | ||||
| 11 | THR | MET | CYS | ILE | ILE | GLU | ASP | ASP | GLU | ILE | ||||
| 12 | LEU | VAL | ARG | TYR | ASP | LYS | THR | LEU | GLU | LYS | ||||
| 13 | LEU | ILE | ASP | GLU | VAL | GLY | VAL | SER | TYR | GLU | ||||
| 14 | GLN | SER | GLU | ARG | ARG | PHE | LYS | GLU | PRO | PHE | ||||
| 15 | LYS | TYR | ARG | GLY | HIS | GLN | HIS |
Samples:
sample_1: Bacillus pasteurii UreE, [U-90% 2H; U-13C; U-15N], 5.6 mM
Ex-cond_1: pH: 6.5; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
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