BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5918

Title: Multiple time-scale dynamics of homologous thermophilic and mesophilic ribonuclease HI enzymes   PubMed: 15165855

Deposition date: 2003-08-21 Original release date: 2004-07-23

Authors: Butterwick, Joel; Patrick, Loria; Astrof, Nathan; Kroenke, Christopher; Cole, Roger; Rance, Mark; Palmer, Arthur

Citation: Butterwick, Joel; Patrick, Loria; Astrof, Nathan; Kroenke, Christopher; Cole, Roger; Rance, Mark; Palmer, Arthur. "Multiple time-scale backbone dynamics of homologous thermophilic and mesophilic ribonuclease HI enzymes"  J. Mol. Biol. 339, 855-871 (2004).

Assembly members:
Ribonuclease HI, polymer, 166 residues, Formula weight is not available

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ribonuclease HI: MNPSPRKRVALFTDGAALGN PGPGGWAALLRFHAHEKLLS GGEASTTNNRMELKAAIEGL KALKEPAEVDLYTDSHYLKK AFTEGWLEGWRKRGWRTAEG KPVKNRDLWEALLLAMAPHR VRFHFVKGHTGHPENERVDR EARRQAQSQAKTPSPPRAPT LFHEEA

Data sets:
Data typeCount
13C chemical shifts439
15N chemical shifts149
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ttRNH monomer1

Entities:

Entity 1, ttRNH monomer 166 residues - Formula weight is not available

1   METASNPROSERPROARGLYSARGVALALA
2   LEUPHETHRASPGLYALAALALEUGLYASN
3   PROGLYPROGLYGLYTRPALAALALEULEU
4   ARGPHEHISALAHISGLULYSLEULEUSER
5   GLYGLYGLUALASERTHRTHRASNASNARG
6   METGLULEULYSALAALAILEGLUGLYLEU
7   LYSALALEULYSGLUPROALAGLUVALASP
8   LEUTYRTHRASPSERHISTYRLEULYSLYS
9   ALAPHETHRGLUGLYTRPLEUGLUGLYTRP
10   ARGLYSARGGLYTRPARGTHRALAGLUGLY
11   LYSPROVALLYSASNARGASPLEUTRPGLU
12   ALALEULEULEUALAMETALAPROHISARG
13   VALARGPHEHISPHEVALLYSGLYHISTHR
14   GLYHISPROGLUASNGLUARGVALASPARG
15   GLUALAARGARGGLNALAGLNSERGLNALA
16   LYSTHRPROSERPROPROARGALAPROTHR
17   LEUPHEHISGLUGLUALA

Samples:

sample_1: Ribonuclease HI, [U-90% 2H; U-96% 13C; U-96% 15N], 0.3 mM; sodium acetate-d3 100 mM; sodium azide 0.02%; D2O 10%

sample_conditions_1: pH: 5.5 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
[1H,15N]-TROSY Triple-Resonancenot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HN(COCA)CBnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 7277
PDB
DBJ BAD71379
EMBL CAA43026
PIR A42673
REF WP_011228761 YP_144822
SP P29253

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts