BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5999

Title: NMR Assignments of the Drosophila Argonaute 2 Paz domain   PubMed: 15213449

Deposition date: 2003-11-07 Original release date: 2004-06-30

Authors: Lingel, Andreas; Simon, Bernd; Izaurralde, Elisa; Sattler, Michael

Citation: Lingel, Andreas; Simon, Bernd; Izaurralde, Elisa; Sattler, Michael. "Letter to the Editor: NMR Assignments of the Drosophila Argonaute 2 PAZ domain"  J. Biomol. NMR 29, 421-422 (2004).

Assembly members:
Argonaute 2 PAZ domain, polymer, 143 residues, 16065 Da.

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Argonaute 2 PAZ domain: GAMAMPMIEYLERFSLKAKI NNTTNLDYSRRFLEPFLRGI NVVYTPPQSFQSAPRVYRVN GLSRAPASSETFEHDGKKVT IASYFHSRNYPLKFPQLHCL NVGSSIKSILLPIELCSIEE GQALNRKDGATQVANMIKYA ATS

Data sets:
Data typeCount
1H chemical shifts898
13C chemical shifts470
15N chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ago2 PAZ domain1

Entities:

Entity 1, Ago2 PAZ domain 143 residues - 16065 Da.

1   GLYALAMETALAMETPROMETILEGLUTYR
2   LEUGLUARGPHESERLEULYSALALYSILE
3   ASNASNTHRTHRASNLEUASPTYRSERARG
4   ARGPHELEUGLUPROPHELEUARGGLYILE
5   ASNVALVALTYRTHRPROPROGLNSERPHE
6   GLNSERALAPROARGVALTYRARGVALASN
7   GLYLEUSERARGALAPROALASERSERGLU
8   THRPHEGLUHISASPGLYLYSLYSVALTHR
9   ILEALASERTYRPHEHISSERARGASNTYR
10   PROLEULYSPHEPROGLNLEUHISCYSLEU
11   ASNVALGLYSERSERILELYSSERILELEU
12   LEUPROILEGLULEUCYSSERILEGLUGLU
13   GLYGLNALALEUASNARGLYSASPGLYALA
14   THRGLNVALALAASNMETILELYSTYRALA
15   ALATHRSER

Samples:

sample_1: Argonaute 2 PAZ domain, [U-100% 13C; U-100% 15N], 1.0 – 1.5 mM

Ex-cond_1: pH: 6.8; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
(H)C-HMQC-NOESY-1H-15N-HSQCnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
H(CCCO)NH-TOCSYnot availablenot availablenot available
(H)C(CCCO)NH-TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHA-Jnot availablenot availablenot available
15N T1, T2, hetNOEnot availablenot availablenot available

Software:

NMRView v5.0.4 - peak assignments

ARIA v1.2 - Structure Calculation

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 700 MHz
  • Bruker DRX 900 MHz

Related Database Links:

PDB
GB AAF49619 AAF49620 AAM11104 AAO39550 ABB54719
REF NP_001261882 NP_648775 NP_730054
SP Q9VUQ5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts