BMRB Entry 5999
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5999
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR Assignments of the Drosophila Argonaute 2 Paz domain PubMed: 15213449
Deposition date: 2003-11-07 Original release date: 2004-06-30
Authors: Lingel, Andreas; Simon, Bernd; Izaurralde, Elisa; Sattler, Michael
Citation: Lingel, Andreas; Simon, Bernd; Izaurralde, Elisa; Sattler, Michael. "Letter to the Editor: NMR Assignments of the Drosophila Argonaute 2 PAZ domain" J. Biomol. NMR 29, 421-422 (2004).
Assembly members:
Argonaute 2 PAZ domain, polymer, 143 residues, 16065 Da.
Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Argonaute 2 PAZ domain: GAMAMPMIEYLERFSLKAKI
NNTTNLDYSRRFLEPFLRGI
NVVYTPPQSFQSAPRVYRVN
GLSRAPASSETFEHDGKKVT
IASYFHSRNYPLKFPQLHCL
NVGSSIKSILLPIELCSIEE
GQALNRKDGATQVANMIKYA
ATS
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 898 |
13C chemical shifts | 470 |
15N chemical shifts | 131 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Ago2 PAZ domain | 1 |
Entities:
Entity 1, Ago2 PAZ domain 143 residues - 16065 Da.
1 | GLY | ALA | MET | ALA | MET | PRO | MET | ILE | GLU | TYR | ||||
2 | LEU | GLU | ARG | PHE | SER | LEU | LYS | ALA | LYS | ILE | ||||
3 | ASN | ASN | THR | THR | ASN | LEU | ASP | TYR | SER | ARG | ||||
4 | ARG | PHE | LEU | GLU | PRO | PHE | LEU | ARG | GLY | ILE | ||||
5 | ASN | VAL | VAL | TYR | THR | PRO | PRO | GLN | SER | PHE | ||||
6 | GLN | SER | ALA | PRO | ARG | VAL | TYR | ARG | VAL | ASN | ||||
7 | GLY | LEU | SER | ARG | ALA | PRO | ALA | SER | SER | GLU | ||||
8 | THR | PHE | GLU | HIS | ASP | GLY | LYS | LYS | VAL | THR | ||||
9 | ILE | ALA | SER | TYR | PHE | HIS | SER | ARG | ASN | TYR | ||||
10 | PRO | LEU | LYS | PHE | PRO | GLN | LEU | HIS | CYS | LEU | ||||
11 | ASN | VAL | GLY | SER | SER | ILE | LYS | SER | ILE | LEU | ||||
12 | LEU | PRO | ILE | GLU | LEU | CYS | SER | ILE | GLU | GLU | ||||
13 | GLY | GLN | ALA | LEU | ASN | ARG | LYS | ASP | GLY | ALA | ||||
14 | THR | GLN | VAL | ALA | ASN | MET | ILE | LYS | TYR | ALA | ||||
15 | ALA | THR | SER |
Samples:
sample_1: Argonaute 2 PAZ domain, [U-100% 13C; U-100% 15N], 1.0 1.5 mM
Ex-cond_1: pH: 6.8; temperature: 295 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-1H NOESY | not available | not available | not available |
1H-15N NOESY | not available | not available | not available |
1H-13C NOESY | not available | not available | not available |
(H)C-HMQC-NOESY-1H-15N-HSQC | not available | not available | not available |
HCCH-TOCSY | not available | not available | not available |
H(CCCO)NH-TOCSY | not available | not available | not available |
(H)C(CCCO)NH-TOCSY | not available | not available | not available |
HNCA | not available | not available | not available |
HNCACB | not available | not available | not available |
CBCA(CO)NH | not available | not available | not available |
HNHA-J | not available | not available | not available |
15N T1, T2, hetNOE | not available | not available | not available |
Software:
NMRView v5.0.4 - peak assignments
ARIA v1.2 - Structure Calculation
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
- Bruker DRX 700 MHz
- Bruker DRX 900 MHz
Related Database Links:
PDB | |
GB | AAF49619 AAF49620 AAM11104 AAO39550 ABB54719 |
REF | NP_001261882 NP_648775 NP_730054 |
SP | Q9VUQ5 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts