BMRB Entry 6003
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6003
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Title: Complete Resonance Assignments of Bundlin (BfpA) from the Bundle- forming Pilus of Enteropathogenic Escherichia coli PubMed: 15213452
Deposition date: 2003-11-11 Original release date: 2004-07-06
Authors: Ramboarina, Stephanie; Fernandes, Paula; Simpson, Peter; Frankel, Gad; Donnenberg, Michael; Matthews, Steve
Citation: Ramboarina, Stephanie; Fernandes, Paula; Simpson, Peter; Frankel, Gad; Donnenberg, Michael; Matthews, Stephen. "Letter to the Editor: Complete Resonance Assignments of Bundlin (BfpA) from the Bundle-forming Pilus of Enteropathogenic Escherichia coli" J. Biomol. NMR 29, 427-428 (2004).
Assembly members:
bundlin, polymer, 164 residues, 18300 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
bundlin: HHHHHHMEQSASDSNKSQNA
ISEVMSATSAINGLYIGQTS
YSGLDSTILLNTSAIPDNYK
DTTNKKITNPFGGELNVGPA
NNNTAFGYYLTLTRLDKAAC
VSLATLNLGTSAKGYGVNIS
GENNITSFGNSADQAAKSTA
ITPAEAATACKNTDSTNKVT
YFMK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 938 |
| 13C chemical shifts | 610 |
| 15N chemical shifts | 148 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | bfpA monomer | 1 |
Entities:
Entity 1, bfpA monomer 164 residues - 18300 Da.
| 1 | HIS | HIS | HIS | HIS | HIS | HIS | MET | GLU | GLN | SER | ||||
| 2 | ALA | SER | ASP | SER | ASN | LYS | SER | GLN | ASN | ALA | ||||
| 3 | ILE | SER | GLU | VAL | MET | SER | ALA | THR | SER | ALA | ||||
| 4 | ILE | ASN | GLY | LEU | TYR | ILE | GLY | GLN | THR | SER | ||||
| 5 | TYR | SER | GLY | LEU | ASP | SER | THR | ILE | LEU | LEU | ||||
| 6 | ASN | THR | SER | ALA | ILE | PRO | ASP | ASN | TYR | LYS | ||||
| 7 | ASP | THR | THR | ASN | LYS | LYS | ILE | THR | ASN | PRO | ||||
| 8 | PHE | GLY | GLY | GLU | LEU | ASN | VAL | GLY | PRO | ALA | ||||
| 9 | ASN | ASN | ASN | THR | ALA | PHE | GLY | TYR | TYR | LEU | ||||
| 10 | THR | LEU | THR | ARG | LEU | ASP | LYS | ALA | ALA | CYS | ||||
| 11 | VAL | SER | LEU | ALA | THR | LEU | ASN | LEU | GLY | THR | ||||
| 12 | SER | ALA | LYS | GLY | TYR | GLY | VAL | ASN | ILE | SER | ||||
| 13 | GLY | GLU | ASN | ASN | ILE | THR | SER | PHE | GLY | ASN | ||||
| 14 | SER | ALA | ASP | GLN | ALA | ALA | LYS | SER | THR | ALA | ||||
| 15 | ILE | THR | PRO | ALA | GLU | ALA | ALA | THR | ALA | CYS | ||||
| 16 | LYS | ASN | THR | ASP | SER | THR | ASN | LYS | VAL | THR | ||||
| 17 | TYR | PHE | MET | LYS |
Samples:
sample_1: bundlin, [U-95% 13C; U-95% 15N], 1 mM
Ex-cond_1: pH: 5.2 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| H(C)CH-TOCSY | not available | not available | not available |
| (H)CC(CO)NH-TOCSY | not available | not available | not available |
| 15N NOESY-HSQC | not available | not available | not available |
| 13C NOESY-HSQC | not available | not available | not available |
Software:
NMRView v5.0.4 -
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA84838 BAE98279 BAE98284 BAE98287 BAE98291 |
| EMBL | CAA78167 CAA92326 CAJ34994 CAS07439 CAY54182 |
| GB | AAC44040 AAG16257 AAG16261 AAG16262 AAG16265 |
| REF | NP_053065 WP_000253757 WP_000253758 YP_001965379 YP_002332157 |
| SP | P33553 P58997 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts