BMRB Entry 6021
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6021
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Title: Backbone 1H, 15N and 13C assignments for the subunit a of the E.coli ATP Synthase PubMed: 15213458
Deposition date: 2003-11-24 Original release date: 2004-07-06
Authors: Dmitriev, Oleg; Abildgaard, Frits; Markley, John; Fillingame, Robert
Citation: Dmitriev, Oleg; Abildgaard, Frits; Markley, John; Fillingame, Robert. "Letter to the Editor: Backbone 1H, 15N and 13C assignments for the subunit a of the E.coli ATP Synthase " J. Biomol. NMR 29, 439-440 (2004).
Assembly members:
subunit a of the ATP synthase, polymer, 277 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
subunit a of the ATP synthase: MHHHHHHASENMTPQDYIGH
HLNNLQLDLRTFSLVDPQNP
PATFWTINIDSMFFSVVLGL
LFLVLFRSVAKKATSGVPGK
FQTAIELVIGFVNGSVKDMY
HGKSKLIAPLALTIFVWVFL
MNLMDLLPIDLLPYIAEHVL
GLPALRVVPSADVNVTLSMA
LGVFILILFYSIKMKGIGGF
TKELTLQPFNHWAFIPVNLI
LEGVSLLSKPVSLGLRLFGN
MYAGELIFILIAGLLPWWSQ
WILNVPWAIFHILIITLQAF
IFMVLTIVYLSMASEEH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 244 |
| 13C chemical shifts | 753 |
| 15N chemical shifts | 244 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | sibunit a | 1 |
Entities:
Entity 1, sibunit a 277 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | ALA | SER | GLU | ||||
| 2 | ASN | MET | THR | PRO | GLN | ASP | TYR | ILE | GLY | HIS | ||||
| 3 | HIS | LEU | ASN | ASN | LEU | GLN | LEU | ASP | LEU | ARG | ||||
| 4 | THR | PHE | SER | LEU | VAL | ASP | PRO | GLN | ASN | PRO | ||||
| 5 | PRO | ALA | THR | PHE | TRP | THR | ILE | ASN | ILE | ASP | ||||
| 6 | SER | MET | PHE | PHE | SER | VAL | VAL | LEU | GLY | LEU | ||||
| 7 | LEU | PHE | LEU | VAL | LEU | PHE | ARG | SER | VAL | ALA | ||||
| 8 | LYS | LYS | ALA | THR | SER | GLY | VAL | PRO | GLY | LYS | ||||
| 9 | PHE | GLN | THR | ALA | ILE | GLU | LEU | VAL | ILE | GLY | ||||
| 10 | PHE | VAL | ASN | GLY | SER | VAL | LYS | ASP | MET | TYR | ||||
| 11 | HIS | GLY | LYS | SER | LYS | LEU | ILE | ALA | PRO | LEU | ||||
| 12 | ALA | LEU | THR | ILE | PHE | VAL | TRP | VAL | PHE | LEU | ||||
| 13 | MET | ASN | LEU | MET | ASP | LEU | LEU | PRO | ILE | ASP | ||||
| 14 | LEU | LEU | PRO | TYR | ILE | ALA | GLU | HIS | VAL | LEU | ||||
| 15 | GLY | LEU | PRO | ALA | LEU | ARG | VAL | VAL | PRO | SER | ||||
| 16 | ALA | ASP | VAL | ASN | VAL | THR | LEU | SER | MET | ALA | ||||
| 17 | LEU | GLY | VAL | PHE | ILE | LEU | ILE | LEU | PHE | TYR | ||||
| 18 | SER | ILE | LYS | MET | LYS | GLY | ILE | GLY | GLY | PHE | ||||
| 19 | THR | LYS | GLU | LEU | THR | LEU | GLN | PRO | PHE | ASN | ||||
| 20 | HIS | TRP | ALA | PHE | ILE | PRO | VAL | ASN | LEU | ILE | ||||
| 21 | LEU | GLU | GLY | VAL | SER | LEU | LEU | SER | LYS | PRO | ||||
| 22 | VAL | SER | LEU | GLY | LEU | ARG | LEU | PHE | GLY | ASN | ||||
| 23 | MET | TYR | ALA | GLY | GLU | LEU | ILE | PHE | ILE | LEU | ||||
| 24 | ILE | ALA | GLY | LEU | LEU | PRO | TRP | TRP | SER | GLN | ||||
| 25 | TRP | ILE | LEU | ASN | VAL | PRO | TRP | ALA | ILE | PHE | ||||
| 26 | HIS | ILE | LEU | ILE | ILE | THR | LEU | GLN | ALA | PHE | ||||
| 27 | ILE | PHE | MET | VAL | LEU | THR | ILE | VAL | TYR | LEU | ||||
| 28 | SER | MET | ALA | SER | GLU | GLU | HIS |
Samples:
sample_1: subunit a of the ATP synthase, [U-13C; U-15N; non-exchangeable 90% 2H], 0.3 mM; CD3COONa 25 mM; CHCl3:CD3OH:H2O (4:4:1) 100%
conditions: pH*: 5.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
| HN(CA)CB | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| HNCACO | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAB38103 BAE77550 BAG79552 BAI27998 BAI33121 |
| EMBL | CAA23514 CAA23520 CAA23521 CAA23590 CAA25776 |
| GB | AAA24731 AAA24740 AAA62090 AAA83869 AAC76761 |
| REF | NP_290377 NP_312707 NP_418194 NP_709551 NP_756524 |
| SP | A1AHR8 A7ZTV0 A8A6K1 B1IX00 B1LL65 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts