BMRB Entry 6031
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6031
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Title: 1H, 13C, and 15N resonance assignments of human Notch-1 calcium binding EGF domains 11-13 PubMed: 15213460
Deposition date: 2003-12-03 Original release date: 2004-07-06
Authors: Muranyi, Andreas; Hambleton, Sophie; Knott, Vroni; McMichael, Andrew; Handford, Penny; Downing, A. Kristina
Citation: Muranyi, Andreas; Hambleton, Sophie; Knott, Vroni; McMichael, Andrew; Handford, Penny; Downing, A.. "Letter to the Editor: 1H, 13C, and 15N resonance assignments of human Notch-1 calcium binding EGF domains 11-13" J. Biomol. NMR 29, 443-444 (2004).
Assembly members:
human Notch-1, polymer, 118 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo Sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
human Notch-1: SAQDVDECSLGANPCEHAGK
CINTLGSFECQCLQGYTGPR
CEIDVNECVSNPCQNDATCL
DQIGEFQCICMPGYEGVHCE
VNTDECASSPCLHNGRCLDK
INEFQCECPTGFTGHLCQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 382 |
| 15N chemical shifts | 118 |
| 1H chemical shifts | 661 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hN11-13 | 1 |
| 2 | Calcium ion | 2 |
| 3 | Calcium ion | 2 |
| 4 | Calcium ion | 2 |
Entities:
Entity 1, hN11-13 118 residues - Formula weight is not available
| 1 | SER | ALA | GLN | ASP | VAL | ASP | GLU | CYS | SER | LEU | ||||
| 2 | GLY | ALA | ASN | PRO | CYS | GLU | HIS | ALA | GLY | LYS | ||||
| 3 | CYS | ILE | ASN | THR | LEU | GLY | SER | PHE | GLU | CYS | ||||
| 4 | GLN | CYS | LEU | GLN | GLY | TYR | THR | GLY | PRO | ARG | ||||
| 5 | CYS | GLU | ILE | ASP | VAL | ASN | GLU | CYS | VAL | SER | ||||
| 6 | ASN | PRO | CYS | GLN | ASN | ASP | ALA | THR | CYS | LEU | ||||
| 7 | ASP | GLN | ILE | GLY | GLU | PHE | GLN | CYS | ILE | CYS | ||||
| 8 | MET | PRO | GLY | TYR | GLU | GLY | VAL | HIS | CYS | GLU | ||||
| 9 | VAL | ASN | THR | ASP | GLU | CYS | ALA | SER | SER | PRO | ||||
| 10 | CYS | LEU | HIS | ASN | GLY | ARG | CYS | LEU | ASP | LYS | ||||
| 11 | ILE | ASN | GLU | PHE | GLN | CYS | GLU | CYS | PRO | THR | ||||
| 12 | GLY | PHE | THR | GLY | HIS | LEU | CYS | GLN |
Entity 2, Calcium ion - Ca - 40.078 Da.
| 1 | CA |
Samples:
15N_sample: human Notch-1, [U-15N], 1.0 mM; CaCl2 15 mM; NaN3 0.02%
13C_15N_sample: human Notch-1, [U-13C; U-15N], 0.5 mM
Ex-cond_1: ionic strength: 0.09 M; pH: 6.1; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N TOCSY-HSQC | not available | not available | Ex-cond_1 |
| 1H-15N NOESY-HSQC | not available | not available | Ex-cond_1 |
| HNCA | not available | not available | Ex-cond_1 |
| HN(C0)CA | not available | not available | Ex-cond_1 |
| HNCACB | not available | not available | Ex-cond_1 |
| CBCA(CO)NH | not available | not available | Ex-cond_1 |
| HNCO | not available | not available | Ex-cond_1 |
| HCCH-TOCSY | not available | not available | Ex-cond_1 |
| 1H-13C NOESY | not available | not available | Ex-cond_1 |
Software:
FELIX v2.3 - Processing
NMRView v5.0 - Analysis
formatconverter v1.0 b2 - Format conversion to NMRStar
NMR spectrometers:
- GE Omega 600 MHz
- GE Omega 750 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
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