BMRB Entry 6094

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6094

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR structure of the 101 nucleotide core encapsidation signal of the moloney murine leukemia virus PubMed: 15003457

Deposition date: 2004-02-06 Original release date: 2004-05-15

Authors: DSouza, Victoria; Dey, Anwesha; Habib, Dina; Summers, Michael

Citation: D'Souza, Victoria; Dey, Anwesha; Habib, Dina; Summers, Michael. "NMR structure of the 101 nucleotide core encapsidation signal of the moloney murine leukemia virus" J. Mol. Biol. 337, 427-442 (2004).

Assembly members:
single chain of RNA, polymer, 101 residues, Formula weight is not available

Natural source: Common Name: Moloney Murine Leukemia Virus Taxonomy ID: 11786 Superkingdom: Viruses Kingdom: not available Genus/species: Gammaretrovirus murine leukemia virus

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
single chain of RNA: GGCGGUACUAGUUGAGAAAC UAGCUCUGUAUCUGGUGGAC CCGUGGUGGAACUGUGAAGU UCGGAACACCCGGCCGCAAC CCUGGGAGAGGUCCCAGGGU U

Data sets:

assigned_chemical_shifts
- set_1

Data type	Count
13C chemical shifts	578
15N chemical shifts	35
1H chemical shifts	683

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	psi site monomer	1

Entities:

Entity 1, psi site monomer 101 residues - Formula weight is not available

1

G

C

G

U

A

C

U

A

2

G

U

G

A

G

A

C

3

U

A

G

C

U

C

U

G

U

A

4

U

C

U

G

U

G

A

C

5

C

G

U

G

U

G

A

6

A

C

U

G

U

G

A

G

U

7

U

C

G

A

C

A

C

8

C

G

C

G

C

A

C

9

C

U

G

A

G

A

G

10

G

U

C

A

G

U

11

U

Samples:

sample_1: single chain of RNA, [13C; 15N]-Ade, 1.2

sample_2: single chain of RNA, [13C; 15N]-Gua, 1.2

sample_3: single chain of RNA, [13C; 15N]-Cyt, 1.2

sample_4: single chain of RNA, [13C; 15N]-Ura, 1.2

sample_5: single chain of RNA, [15N]-Gua-Ura, 1.2

sample_6: single chain of RNA, [2D]-Ade-Ura-Cyt, 1.2

sample_7: single chain of RNA, [2D]-Ade-Gua-Ura, 1.2

sample_8: single chain of RNA, [2D]-Ade-Gua-Cyt, 1.2

sample_9: single chain of RNA, [2D]-Gua-Cyt-Ura, 1.2

cond-1: pH: 7.0; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	cond-1
2D ROESY	not available	not available	cond-1
2D HMQC	not available	not available	cond-1
3D HMQC-NOESY	not available	not available	cond-1
4d-HMQC NOESY HMQC	not available	not available	cond-1
2D 1H-15N HSQC	not available	not available	cond-1
3D HSQC-NOESY	not available	not available	cond-1

Software:

No software information available

NMR spectrometers:

Bruker . 800 MHz
Bruker . 600 MHz