BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 6184

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6184

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Title: Model for the Catalytic Domain of the Proofreading Epsilon Subunit of E.coli DNA Polymerase III Based on NMR Structural Data   PubMed: 11772007

Deposition date: 2004-04-20 Original release date: 2004-06-25

Authors: DeRose, Eugene; Li, Dawei; Darden, Thomas; Harvey, Scott; Perrino, Fred; Schaaper, Roel

Citation: DeRose, Eugene. "Model for the Catalytic Domain of the Proofreading Epsilon Subunit of Escherichia coli DNA Polymerase III Based on NMR Structural Data"  Biochemistry 41, 94-110 (2002).

Assembly members:
epsilon-186, polymer, 186 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
epsilon-186: MSTAITRQIVLDTETTGMNQ IGAHYEGHKIIEIGAVEVVN RRLTGNNFHVYLKPDRLVDP EAFGVHGIADEFLLDKPTFA EVADEFMDYIRGAELVIHNA AFDIGFMDYEFSLLKRDIPK TNTFCKVTDSLAVARKMFPG KRNSLDALCARYEIDNSKRT LHGALLDAQILAEVYLAMTG GQTSMA

Data sets:
Data typeCount
1H chemical shifts361
13C chemical shifts534
15N chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain of the epsilon subunit of E. coli DNA polymerase III1

Entities:

Entity 1, N-terminal domain of the epsilon subunit of E. coli DNA polymerase III 186 residues - Formula weight is not available

1   METSERTHRALAILETHRARGGLNILEVAL
2   LEUASPTHRGLUTHRTHRGLYMETASNGLN
3   ILEGLYALAHISTYRGLUGLYHISLYSILE
4   ILEGLUILEGLYALAVALGLUVALVALASN
5   ARGARGLEUTHRGLYASNASNPHEHISVAL
6   TYRLEULYSPROASPARGLEUVALASPPRO
7   GLUALAPHEGLYVALHISGLYILEALAASP
8   GLUPHELEULEUASPLYSPROTHRPHEALA
9   GLUVALALAASPGLUPHEMETASPTYRILE
10   ARGGLYALAGLULEUVALILEHISASNALA
11   ALAPHEASPILEGLYPHEMETASPTYRGLU
12   PHESERLEULEULYSARGASPILEPROLYS
13   THRASNTHRPHECYSLYSVALTHRASPSER
14   LEUALAVALALAARGLYSMETPHEPROGLY
15   LYSARGASNSERLEUASPALALEUCYSALA
16   ARGTYRGLUILEASPASNSERLYSARGTHR
17   LEUHISGLYALALEULEUASPALAGLNILE
18   LEUALAGLUVALTYRLEUALAMETTHRGLY
19   GLYGLNTHRSERMETALA

Samples:

sample_1: epsilon-186, [U-2H; U-13C; U-15N], 0.72 mM

Ex-cond_1: pH: 7.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HN(CA)CBsample_1not availableEx-cond_1
HN(COCA)CBsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
H(CCO)NH-TOCSYsample_1not availableEx-cond_1
(H)C(CO)NH-TOCSYsample_1not availableEx-cond_1
3D 15N-separated NOESYsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
DBJ BAA77886 BAB33634 BAG75735 BAI23571 BAI29085
EMBL CAA27661 CAP74779 CAQ30730 CAQ87813 CAQ97098
GB AAA24564 AAB08637 AAC73320 AAG54511 AAN41863
REF NP_285903 NP_308238 NP_414751 NP_706156 NP_752198
SP P03007

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts