BMRB Entry 6223
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6223
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Title: Sequential resonance assignment of yeast cytosine deaminase in complex with a transition state analogue PubMed: 17091334
Deposition date: 2004-06-02 Original release date: 2007-03-22
Authors: Yao, Lishan; Liu, Aizhuo; Li, Yue; Felczak, Krzysztof; Li, Guangyu; Yan, Honggao
Citation: Liu, Aizhuo; Li, Yue; Yao, L.; Yan, Honggao. "Simultaneous NMR assignment of backbone and side chain amides in large proteins with IS-TROSY" J. Biomol. NMR 36, 205-214 (2006).
Assembly members:
Yeast cytosine deaminase, polymer, 158 residues, Formula weight is not available
5-fluorine, 2-hydroxypyrimidine, non-polymer, 114.0778232 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Yeast cytosine deaminase: MVTGGMASKWDQKGMDIAYE
EAALGYKEGGVPIGGCLINN
KDGSVLGRGHNMRFQKGSAT
LHGEISTLENCGRLEGKVYK
DTTLYTTLSPCDMCTGAIIM
YGIPRCVVGENVNFKSKGEK
YLQTRGHEVVVVDDERCKKI
MKQFIDERPQDWFEDIGE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 308 |
| 13C chemical shifts | 424 |
| 15N chemical shifts | 145 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YCD monomer 1 | 1 |
| 2 | YCD monomer 2 | 1 |
| 3 | 5-fluorine, 2-hydroxypyrimidine | 2 |
| 4 | ZINC (II) ION | 3 |
Entities:
Entity 1, YCD monomer 1 158 residues - Formula weight is not available
| 1 | MET | VAL | THR | GLY | GLY | MET | ALA | SER | LYS | TRP | ||||
| 2 | ASP | GLN | LYS | GLY | MET | ASP | ILE | ALA | TYR | GLU | ||||
| 3 | GLU | ALA | ALA | LEU | GLY | TYR | LYS | GLU | GLY | GLY | ||||
| 4 | VAL | PRO | ILE | GLY | GLY | CYS | LEU | ILE | ASN | ASN | ||||
| 5 | LYS | ASP | GLY | SER | VAL | LEU | GLY | ARG | GLY | HIS | ||||
| 6 | ASN | MET | ARG | PHE | GLN | LYS | GLY | SER | ALA | THR | ||||
| 7 | LEU | HIS | GLY | GLU | ILE | SER | THR | LEU | GLU | ASN | ||||
| 8 | CYS | GLY | ARG | LEU | GLU | GLY | LYS | VAL | TYR | LYS | ||||
| 9 | ASP | THR | THR | LEU | TYR | THR | THR | LEU | SER | PRO | ||||
| 10 | CYS | ASP | MET | CYS | THR | GLY | ALA | ILE | ILE | MET | ||||
| 11 | TYR | GLY | ILE | PRO | ARG | CYS | VAL | VAL | GLY | GLU | ||||
| 12 | ASN | VAL | ASN | PHE | LYS | SER | LYS | GLY | GLU | LYS | ||||
| 13 | TYR | LEU | GLN | THR | ARG | GLY | HIS | GLU | VAL | VAL | ||||
| 14 | VAL | VAL | ASP | ASP | GLU | ARG | CYS | LYS | LYS | ILE | ||||
| 15 | MET | LYS | GLN | PHE | ILE | ASP | GLU | ARG | PRO | GLN | ||||
| 16 | ASP | TRP | PHE | GLU | ASP | ILE | GLY | GLU |
Entity 2, 5-fluorine, 2-hydroxypyrimidine 1 residues - C4 H3 N2 O F - 114.0778232 Da.
| 1 | PYR1 |
Entity 3, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Yeast cytosine deaminase, [U-70% 2H, U-100% 13C, U-100% 15N], 1.8 mM
Ex-cond_1: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_1 | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| REF | NP_015387 |
| SWISS-PROT | Q12178 |
| GenBank | EDN61192 EDV11264 AAC13409 AAG33626 AAB67713 |
| EMBL | CAA95006 CAA89179 |
| PDB |
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