BMRB Entry 6256

Name: Chemical Shift Assignments of TM1509
Published: 2005-08-22

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6256

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Chemical Shift Assignments of TM1509

Deposition date: 2004-06-29 Original release date: 2005-08-22

Authors: Herve du Penhoat, Catherine; Atreya, Hanudatta; Kim, Seho; Yee, Adelinda; Xiao, Rong; Arrowsmith, Cheryl; Szyperski, Thomas

Citation: Herve du Penhoat, Catherine; Li, Zhaohui; Atreya, Hanudatta; Kim, Seho; Yee, Adelinda; Xiao, Rong; Murray, Diana; Arrowsmith, Cheryl; Szyperski, Thomas. "NMR solution structure of Thermotoga maritima protein TM1509 reveals a Zn-metalloprotease-like tertiary structure " J. Struct. Funct. Genomics 6, 51-62 (2005).

Assembly members:
TM1509, polymer, 150 residues, 17549.8 Da.

Natural source: Common Name: Thermotoga maritima Taxonomy ID: 2336 Superkingdom: Eubacteria Kingdom: not available Genus/species: Thermotoga maritima

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
TM1509: MIRILGEGKGSKLLENLKEK LEEIVKKEIGDVHVNVILVS EDEIKELNQQFRGQDRPTDV LTFPLMEEDVYGEIYVCPLI VEENAREFNNTFEKELLEVV IHGILHLAGYDHEFEDKNSK EMFEKQKKYVEEVWGEWRSN PSEDSDPGKR

Data sets:

assigned_chemical_shifts
- Chemical_shift_ref_1

Data type	Count
1H chemical shifts	1050
13C chemical shifts	624
15N chemical shifts	154

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TM1509	1

Entities:

Entity 1, TM1509 150 residues - 17549.8 Da.

1	MET	ILE	ARG	ILE	LEU	GLY	GLU	GLY	LYS	GLY
2	SER	LYS	LEU	LEU	GLU	ASN	LEU	LYS	GLU	LYS
3	LEU	GLU	GLU	ILE	VAL	LYS	LYS	GLU	ILE	GLY
4	ASP	VAL	HIS	VAL	ASN	VAL	ILE	LEU	VAL	SER
5	GLU	ASP	GLU	ILE	LYS	GLU	LEU	ASN	GLN	GLN
6	PHE	ARG	GLY	GLN	ASP	ARG	PRO	THR	ASP	VAL
7	LEU	THR	PHE	PRO	LEU	MET	GLU	GLU	ASP	VAL
8	TYR	GLY	GLU	ILE	TYR	VAL	CYS	PRO	LEU	ILE
9	VAL	GLU	GLU	ASN	ALA	ARG	GLU	PHE	ASN	ASN
10	THR	PHE	GLU	LYS	GLU	LEU	LEU	GLU	VAL	VAL
11	ILE	HIS	GLY	ILE	LEU	HIS	LEU	ALA	GLY	TYR
12	ASP	HIS	GLU	PHE	GLU	ASP	LYS	ASN	SER	LYS
13	GLU	MET	PHE	GLU	LYS	GLN	LYS	LYS	TYR	VAL
14	GLU	GLU	VAL	TRP	GLY	GLU	TRP	ARG	SER	ASN
15	PRO	SER	GLU	ASP	SER	ASP	PRO	GLY	LYS	ARG

Samples:

Sample_1: TM1509, [U-100% 13C; U-100% 15N], 1.0 mM; NaCl 450 mM; NaHPO4 25 mM; DTT 10 mM; NaN3 0.01%; benzamidine 1 mM; inhibitor cocktail pill 1 mM

Sample_2: TM1509, [U-100% 15N; biosynthetically-directed 5% fractionally-labeled 13C], 1.23 mM; NaCl 450 mM; NaHPO4 25 mM; DTT 10 mM; NaN3 0.01%; benzamidine 1 mM; inhibitor cocktail pill 1 mM

all_conditions: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
gNhsqc	not available	not available	not available
CT-gChsqc	not available	not available	not available
HNCO	not available	not available	not available
HNCACB	not available	not available	not available
CBCAcoNH	not available	not available	not available
HNcaHA	not available	not available	not available
HCCHCOSY [13C,1H,1H]	not available	not available	not available
HCCHTOCSY [13C,13C,1H]	not available	not available	not available
13C-resolved aliphatic NOESY	not available	not available	not available
13C-resolved aromatic NOESY	not available	not available	not available
15N-resolved NOESY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian INOVA 750 MHz
Varian INOVA 750 MHz

PDB	1TVI
GB	AAD36576 ACB09747 AGL50441 AHD18596 AIY86860
REF	NP_229309 WP_004081854 WP_008194976 WP_012311098 YP_001739430
SP	B1LBP9 Q9X1J7

Biological Magnetic Resonance Data Bank