BMRB Entry 6319
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6319
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Nkx2.5 Homeodomain plus NK2 Specific Domain in the DNA Bound State
Deposition date: 2004-09-21 Original release date: .
Authors: Gruschus, James; Lee, Hyun-Sook; Ferretti, James
Citation: Lee, Hyun-Sook; Gruschus, James; Zhang, Tao; Ferretti, James. "Letter to the Editor: NMR assignments of the DNA-bound human Csx/Nkx2.5 homeodomain and NK2-specific domain" J. Biomol. NMR 31, 75-76 (2005).
Assembly members:
nkx2.5 homeodomain plus NK2 specific domain, polymer, 152 residues, Formula weight is not available
DNA, polymer, 16 residues, Formula weight is not available
DNA, polymer, 16 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Animalia Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
nkx2.5 homeodomain plus NK2 specific domain: MHHHHHHSSGLVPRGSGMKE
TAAAKFERQHMDSPDLGTDD
DDKAMAERPRARRRRKPRVL
FSQAQVYELERRFKQQRYLS
APERDQLASVLKLTSTQVKI
WFQNRRYKCKRQRQDQTLEL
VGLPPPPPPPARRIAVPVLV
RDGKPCLGDSAP
DNA: TGTGTCAAGTGGCTGT
DNA: ACAGCCACTTGACACA
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 255 |
13C chemical shifts | 169 |
15N chemical shifts | 134 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | nkx2.5 | 1 |
2 | DNA strand 1 | 2 |
3 | DNA strand 2 | 3 |
Entities:
Entity 1, nkx2.5 152 residues - Formula weight is not available
1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | SER | SER | GLY | ||||
2 | LEU | VAL | PRO | ARG | GLY | SER | GLY | MET | LYS | GLU | ||||
3 | THR | ALA | ALA | ALA | LYS | PHE | GLU | ARG | GLN | HIS | ||||
4 | MET | ASP | SER | PRO | ASP | LEU | GLY | THR | ASP | ASP | ||||
5 | ASP | ASP | LYS | ALA | MET | ALA | GLU | ARG | PRO | ARG | ||||
6 | ALA | ARG | ARG | ARG | ARG | LYS | PRO | ARG | VAL | LEU | ||||
7 | PHE | SER | GLN | ALA | GLN | VAL | TYR | GLU | LEU | GLU | ||||
8 | ARG | ARG | PHE | LYS | GLN | GLN | ARG | TYR | LEU | SER | ||||
9 | ALA | PRO | GLU | ARG | ASP | GLN | LEU | ALA | SER | VAL | ||||
10 | LEU | LYS | LEU | THR | SER | THR | GLN | VAL | LYS | ILE | ||||
11 | TRP | PHE | GLN | ASN | ARG | ARG | TYR | LYS | CYS | LYS | ||||
12 | ARG | GLN | ARG | GLN | ASP | GLN | THR | LEU | GLU | LEU | ||||
13 | VAL | GLY | LEU | PRO | PRO | PRO | PRO | PRO | PRO | PRO | ||||
14 | ALA | ARG | ARG | ILE | ALA | VAL | PRO | VAL | LEU | VAL | ||||
15 | ARG | ASP | GLY | LYS | PRO | CYS | LEU | GLY | ASP | SER | ||||
16 | ALA | PRO |
Entity 2, DNA strand 1 16 residues - Formula weight is not available
1 | DT | DG | DT | DG | DT | DC | DA | DA | DG | DT | ||||
2 | DG | DG | DC | DT | DG | DT |
Entity 3, DNA strand 2 16 residues - Formula weight is not available
1 | DA | DC | DA | DG | DC | DC | DA | DC | DT | DT | ||||
2 | DG | DA | DC | DA | DC | DA |
Samples:
sample: nkx2.5 homeodomain plus NK2 specific domain, [U-13C; U-15N], 0.4 mM
sample_conditions: pH: 5; temperature: 307 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
not available | sample | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
GB | AAI14090 AAS99114 ABB00236 ABC71311 EHB08399 |
REF | NP_001010959 NP_001039908 XP_001096796 XP_002710431 XP_002744612 |
TPG | DAA18041 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts