BMRB Entry 6439
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6439
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 15N and 13C and side-chain 1H and 13C assignments of the rice phytochrome B PAS1 domain
Deposition date: 2004-12-21 Original release date: 2005-04-08
Authors: Kobayashi, Toshitatsu; Mishima, Masaki; Akagi, Kayo; Sakai, Nobuya; Katoh, Etsuko; Takano, Makoto; Yamazaki, Toshimasa; Kojima, Chojiro
Citation: Kobayashi, Toshitatsu; Mishima, Masaki; Akagi, Kayo; Sakai, Nobuya; Katoh, Etsuko; Takano, Makoto; Yamazaki, Toshimasa; Kojima, Chojiro. "Letter to the Editor: 1H, 15N and 13C backbone and side-chain assignments of the rice phytochrome B PAS1 domain and backbone assignments of the PAS1-PAS2 domain" J. Biomol. NMR 31, 269-270 (2005).
Assembly members:
Rice phytochrome B PAS1 domain, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: Rice Taxonomy ID: 4530 Superkingdom: Eukaryota Kingdom: Plantae Genus/species: Oryza sativa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Rice phytochrome B PAS1 domain: VRLIETATVPIFAVDTDGCI
NGWNAKVAELTGLSVEEAMG
KSLVNDLIFKESEETVNKLL
SRALRGDEDKNVEIKLKTFG
PEQSKGPIFVIVNACSSRDY
TKNIVGVCFVGQDVTGQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 459 |
| 15N chemical shifts | 114 |
| 1H chemical shifts | 754 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rice phytochrome B PAS1 domain | 1 |
Entities:
Entity 1, Rice phytochrome B PAS1 domain 117 residues - Formula weight is not available
| 1 | VAL | ARG | LEU | ILE | GLU | THR | ALA | THR | VAL | PRO | ||||
| 2 | ILE | PHE | ALA | VAL | ASP | THR | ASP | GLY | CYS | ILE | ||||
| 3 | ASN | GLY | TRP | ASN | ALA | LYS | VAL | ALA | GLU | LEU | ||||
| 4 | THR | GLY | LEU | SER | VAL | GLU | GLU | ALA | MET | GLY | ||||
| 5 | LYS | SER | LEU | VAL | ASN | ASP | LEU | ILE | PHE | LYS | ||||
| 6 | GLU | SER | GLU | GLU | THR | VAL | ASN | LYS | LEU | LEU | ||||
| 7 | SER | ARG | ALA | LEU | ARG | GLY | ASP | GLU | ASP | LYS | ||||
| 8 | ASN | VAL | GLU | ILE | LYS | LEU | LYS | THR | PHE | GLY | ||||
| 9 | PRO | GLU | GLN | SER | LYS | GLY | PRO | ILE | PHE | VAL | ||||
| 10 | ILE | VAL | ASN | ALA | CYS | SER | SER | ARG | ASP | TYR | ||||
| 11 | THR | LYS | ASN | ILE | VAL | GLY | VAL | CYS | PHE | VAL | ||||
| 12 | GLY | GLN | ASP | VAL | THR | GLY | GLN |
Samples:
sample_1: Rice phytochrome B PAS1 domain, [U-13C; U-15N], 0.8 mM; potassium phosphate 50 mM; potassium chloride 20 mM; dithiothreitol 5 mM; deuterium oxide, [U-2H], 10%
conditions_1: ionic strength: 0.05 M; pH: 6.8; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 3D-HNCACB | not available | not available | not available |
| 3D-HN(CO)CACB | not available | not available | not available |
| 3D-HNCO | not available | not available | not available |
| 3D-HN(CA)CO | not available | not available | not available |
| 3D-C(CO)NH | not available | not available | not available |
| 3D-H(CCO)NH | not available | not available | not available |
| 4D-HC(CO)NH | not available | not available | not available |
| 3D-HCCH-TOCSY | not available | not available | not available |
| 15N-edited TOCSY-HSQC | not available | not available | not available |
| 2D-NOESY | not available | not available | not available |
Software:
xwinnmr v3.5 - data aquisition
NMRPipe - data processing
SPARKY v3.106 - assignments, peak picking
NMR spectrometers:
- Bruker AVANCE 500 MHz
- Bruker DRX 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts