BMRB Entry 6440
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6440
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Title: 1H, 15N and 13C backbone assignments of 28kD phytochrome B PAS1-PAS2 domain
Deposition date: 2004-12-21 Original release date: 2005-04-08
Authors: Kobayashi, Toshitatsu; Mishima, Masaki; Akagi, Kayo; Sakai, Nobuya; Katoh, Etsuko; Takano, Makoto; Yamazaki, Toshimasa; Kojima, Chojiro
Citation: Kobayashi, Toshitatsu; Mishima, Masaki; Akagi, Kayo; Sakai, Nobuya; Katoh, Etsuko; Takano, Makoto; Yamazaki, Toshimasa; Kojima, Chojiro. "Letter to the Editor: 1H, 15N and 13C backbone and side-chain assignments of the rice phytochrome B PAS1 domain and backbone assignments of the PAS1-PAS2 domain" J. Biomol. NMR 31, 269-270 (2005).
Assembly members:
Rice phytochrome B PAS1-PAS2 domain, polymer, 258 residues, Formula weight is not available
Natural source: Common Name: Rice Taxonomy ID: 4530 Superkingdom: Eukaryota Kingdom: Plantae Genus/species: Oryza sativa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Rice phytochrome B PAS1-PAS2 domain: VRLIETATVPIFAVDTDGCI
NGWNAKVAELTGLSVEEAMG
KSLVNDLIFKESEETVNKLL
SRALRGDEDKNVEIKLKTFG
PEQSKGPIFVIVNACSSRDY
TKNIVGVCFVGQDVTGQKVV
MDKFINIQGDYKAIVHNPNP
LIPPIFASDENTCCSEWNTA
MEKLTGWSRGEVVGKLLVGE
VFGNCCRLKGPDALTKFMIV
LHNAIGGQDCEKFPFSFFDK
NGKYVQALLTANTRSRMDGE
AIGAFCFLQIASPELQQA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 742 |
| 15N chemical shifts | 246 |
| 1H chemical shifts | 246 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rice phytochrome B PAS1-PAS2 domain | 1 |
Entities:
Entity 1, Rice phytochrome B PAS1-PAS2 domain 258 residues - Formula weight is not available
| 1 | VAL | ARG | LEU | ILE | GLU | THR | ALA | THR | VAL | PRO | ||||
| 2 | ILE | PHE | ALA | VAL | ASP | THR | ASP | GLY | CYS | ILE | ||||
| 3 | ASN | GLY | TRP | ASN | ALA | LYS | VAL | ALA | GLU | LEU | ||||
| 4 | THR | GLY | LEU | SER | VAL | GLU | GLU | ALA | MET | GLY | ||||
| 5 | LYS | SER | LEU | VAL | ASN | ASP | LEU | ILE | PHE | LYS | ||||
| 6 | GLU | SER | GLU | GLU | THR | VAL | ASN | LYS | LEU | LEU | ||||
| 7 | SER | ARG | ALA | LEU | ARG | GLY | ASP | GLU | ASP | LYS | ||||
| 8 | ASN | VAL | GLU | ILE | LYS | LEU | LYS | THR | PHE | GLY | ||||
| 9 | PRO | GLU | GLN | SER | LYS | GLY | PRO | ILE | PHE | VAL | ||||
| 10 | ILE | VAL | ASN | ALA | CYS | SER | SER | ARG | ASP | TYR | ||||
| 11 | THR | LYS | ASN | ILE | VAL | GLY | VAL | CYS | PHE | VAL | ||||
| 12 | GLY | GLN | ASP | VAL | THR | GLY | GLN | LYS | VAL | VAL | ||||
| 13 | MET | ASP | LYS | PHE | ILE | ASN | ILE | GLN | GLY | ASP | ||||
| 14 | TYR | LYS | ALA | ILE | VAL | HIS | ASN | PRO | ASN | PRO | ||||
| 15 | LEU | ILE | PRO | PRO | ILE | PHE | ALA | SER | ASP | GLU | ||||
| 16 | ASN | THR | CYS | CYS | SER | GLU | TRP | ASN | THR | ALA | ||||
| 17 | MET | GLU | LYS | LEU | THR | GLY | TRP | SER | ARG | GLY | ||||
| 18 | GLU | VAL | VAL | GLY | LYS | LEU | LEU | VAL | GLY | GLU | ||||
| 19 | VAL | PHE | GLY | ASN | CYS | CYS | ARG | LEU | LYS | GLY | ||||
| 20 | PRO | ASP | ALA | LEU | THR | LYS | PHE | MET | ILE | VAL | ||||
| 21 | LEU | HIS | ASN | ALA | ILE | GLY | GLY | GLN | ASP | CYS | ||||
| 22 | GLU | LYS | PHE | PRO | PHE | SER | PHE | PHE | ASP | LYS | ||||
| 23 | ASN | GLY | LYS | TYR | VAL | GLN | ALA | LEU | LEU | THR | ||||
| 24 | ALA | ASN | THR | ARG | SER | ARG | MET | ASP | GLY | GLU | ||||
| 25 | ALA | ILE | GLY | ALA | PHE | CYS | PHE | LEU | GLN | ILE | ||||
| 26 | ALA | SER | PRO | GLU | LEU | GLN | GLN | ALA |
Samples:
sample_1: Rice phytochrome B PAS1-PAS2 domain, [U-13C; U-15N], 0.2 mM; potassium phosphate 50 mM; potassium chloride 20 mM; dithiothreitol 5 mM; deuterium oxide, [U-2H], 10%
conditions_1: ionic strength: 0.05 M; pH: 6.8; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N_HSQC | not available | not available | not available |
| 3D-HNCACB | not available | not available | not available |
| 3D-HN(CO)CACB | not available | not available | not available |
| 3D-HNCO | not available | not available | not available |
| 3D-HN(CA)CO | not available | not available | not available |
Software:
xwinnmr v3.5 - data aquisition
NMRPipe - data processing
SPARKY v3.106 - assignments, peak picking
NMR spectrometers:
- Bruker AVANCE 500 MHz
Related Database Links:
| DBJ | BAC76432 BAD86669 BAF11824 |
| EMBL | CAA40795 |
| GB | ABF95558 AFK30970 AFK30971 AFK30972 AFK30973 |
| REF | NP_001049910 |
| SP | A2XFW2 Q10MG9 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts