BMRB Entry 6528
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6528
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Title: 1H, 15N and 13C Assignments for the R2 Domain of Choline Binding Protein A (CbpA)
Deposition date: 2005-03-02 Original release date: 2005-08-22
Authors: Luo, Rensheng; Kriwacki, Richard; Mann, Beth; Tuomanen, Elaine
Citation: Luo, Rensheng; Mann, Beth; Tuomanen, Elaine; Kriwacki, Richard. "NMR assignment of the R2 domain of pneumococcal choline binding protein A (CbpA)" J. Biomol. NMR 31, 93-93 (2005).
Assembly members:
Choline binding protein A R2 domain, polymer, 121 residues, 13580 Da.
Natural source: Common Name: not applicable Taxonomy ID: not available Superkingdom: Other Kingdom: Not applicable Genus/species: not applicable not applicable
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Choline binding protein A R2 domain: GSHMPEKKVAEAEKKVEEAK
KKAEDQKEEDRRNYPTNTYK
TLELEIAESDVEVKKAELEL
VKEEAKEPRNEEKVKQAKAE
VESKKAEATRLEKIKTDRKK
AEEEAKRKAAEEDKVKEKPA
E
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 563 |
| 15N chemical shifts | 119 |
| 1H chemical shifts | 862 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Choline_binding_protein_A_R2_domain | 1 |
Entities:
Entity 1, Choline_binding_protein_A_R2_domain 121 residues - 13580 Da.
| 1 | GLY | SER | HIS | MET | PRO | GLU | LYS | LYS | VAL | ALA | ||||
| 2 | GLU | ALA | GLU | LYS | LYS | VAL | GLU | GLU | ALA | LYS | ||||
| 3 | LYS | LYS | ALA | GLU | ASP | GLN | LYS | GLU | GLU | ASP | ||||
| 4 | ARG | ARG | ASN | TYR | PRO | THR | ASN | THR | TYR | LYS | ||||
| 5 | THR | LEU | GLU | LEU | GLU | ILE | ALA | GLU | SER | ASP | ||||
| 6 | VAL | GLU | VAL | LYS | LYS | ALA | GLU | LEU | GLU | LEU | ||||
| 7 | VAL | LYS | GLU | GLU | ALA | LYS | GLU | PRO | ARG | ASN | ||||
| 8 | GLU | GLU | LYS | VAL | LYS | GLN | ALA | LYS | ALA | GLU | ||||
| 9 | VAL | GLU | SER | LYS | LYS | ALA | GLU | ALA | THR | ARG | ||||
| 10 | LEU | GLU | LYS | ILE | LYS | THR | ASP | ARG | LYS | LYS | ||||
| 11 | ALA | GLU | GLU | GLU | ALA | LYS | ARG | LYS | ALA | ALA | ||||
| 12 | GLU | GLU | ASP | LYS | VAL | LYS | GLU | LYS | PRO | ALA | ||||
| 13 | GLU |
Samples:
sample_1: Choline_binding_protein_A_R2_domain, [U-2H; U-13C; U-15N], 1.8 mM; sodium phosphate buffer 10 mM; sodium azide 0.02%; D2O 10%
conditions_1: pH: 5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | isotropic | conditions_1 |
| 3D CT-HNCA, CT-HN(CO)CA | not available | not available | not available |
| 3D TROSY-HNCO, TROSY-HN(CA)CO | not available | not available | not available |
| 3D TROSY-HNCACB, TROSY-HN(CO)CACB | not available | not available | not available |
| 4D 15N/15N-NOESY, | not available | not available | not available |
| 4D TROSY-HNCOCA, TROSY-HNCACO | not available | not available | not available |
| 3D HCCH-TOCSY, HCCH-COSY | not available | not available | not available |
| C(CO)NH-TOCSY,HBHA(CBCACO)NH | not available | not available | not available |
| 3D 13C and 15N-NOESY-HSQC, | not available | not available | not available |
| 4D 13C/13C and 13C/15N NOESY | not available | not available | not available |
Software:
FELIX v2000, Accelerys - analysis NMR data
NMRPipe - process NMR data
NMR spectrometers:
- bruker Avance 800MHz MHz
- varian INOVA 600MHz MHz
- Varian INOVA 900MHz MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts