BMRB Entry 6540
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6540
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Sequence-specific 1H, 13C and 15N resonance assignments of the winged-helix domain of the human Werner syndrome protein
Deposition date: 2005-03-07 Original release date: 2005-08-29
Authors: Sun, Jian-Zhong; Feng, Han-Qiao; Lin, Guang-Xin; Zeng, Wang-Yong; Hu, Jin-Shan
Citation: Sun, Jian-Zhong; Feng, Han-Qiao; Lin, Guang-Xin; Zeng, Wangyong; Hu, Jin-Shan. "NMR Assignments of the Winged-Helix Domain of Human Werner Syndrome Protein" J. Biomol. NMR 32, 261-261 (2005).
Assembly members:
The winged-helix domain of WRN, polymer, 144 residues, 16333.65 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
The winged-helix domain of WRN: MDDSEDTSWDFGPQAFKLLS
AVDILGEKFGIGLPILFLRG
SNSQRLADQYRRHSLFGTGK
DQTESWWKAFSRQLITGGFL
VEVSRYNKFMKICALTKKGR
NWLHKANTESQSLILQANEE
LCPKKLLLPSSKTVSSGTKE
HCYN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 782 |
| 13C chemical shifts | 604 |
| 15N chemical shifts | 149 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | The winged-helix domain of WRN protein | 1 |
Entities:
Entity 1, The winged-helix domain of WRN protein 144 residues - 16333.65 Da.
| 1 | MET | ASP | ASP | SER | GLU | ASP | THR | SER | TRP | ASP | ||||
| 2 | PHE | GLY | PRO | GLN | ALA | PHE | LYS | LEU | LEU | SER | ||||
| 3 | ALA | VAL | ASP | ILE | LEU | GLY | GLU | LYS | PHE | GLY | ||||
| 4 | ILE | GLY | LEU | PRO | ILE | LEU | PHE | LEU | ARG | GLY | ||||
| 5 | SER | ASN | SER | GLN | ARG | LEU | ALA | ASP | GLN | TYR | ||||
| 6 | ARG | ARG | HIS | SER | LEU | PHE | GLY | THR | GLY | LYS | ||||
| 7 | ASP | GLN | THR | GLU | SER | TRP | TRP | LYS | ALA | PHE | ||||
| 8 | SER | ARG | GLN | LEU | ILE | THR | GLY | GLY | PHE | LEU | ||||
| 9 | VAL | GLU | VAL | SER | ARG | TYR | ASN | LYS | PHE | MET | ||||
| 10 | LYS | ILE | CYS | ALA | LEU | THR | LYS | LYS | GLY | ARG | ||||
| 11 | ASN | TRP | LEU | HIS | LYS | ALA | ASN | THR | GLU | SER | ||||
| 12 | GLN | SER | LEU | ILE | LEU | GLN | ALA | ASN | GLU | GLU | ||||
| 13 | LEU | CYS | PRO | LYS | LYS | LEU | LEU | LEU | PRO | SER | ||||
| 14 | SER | LYS | THR | VAL | SER | SER | GLY | THR | LYS | GLU | ||||
| 15 | HIS | CYS | TYR | ASN |
Samples:
sample_1: The winged-helix domain of WRN, [U-95% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%
sample_2: The winged-helix domain of WRN, [U-98% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%
sample_3: The winged-helix domain of WRN, [U-95% 13C; U-95% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%
sample_4: The winged-helix domain of WRN 0.29 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%
Ex-cond_1: pH: 7.36; temperature: 295 K; ionic strength: 0.025 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | Ex-cond_1 |
| HNCACB | not available | not available | Ex-cond_1 |
| CBCA(CO)NH | not available | not available | Ex-cond_1 |
| HNCO | not available | not available | Ex-cond_1 |
| HNCOCO | not available | not available | Ex-cond_1 |
| HNCG_arom | not available | not available | Ex-cond_1 |
| HN(CO)CG_arom | not available | not available | Ex-cond_1 |
| HNCG | not available | not available | Ex-cond_1 |
| HN(CO)C | not available | not available | Ex-cond_1 |
| HNHA | not available | not available | Ex-cond_1 |
| HBHA(CO)NH | not available | not available | Ex-cond_1 |
| DIPSI-H(CCO)NH | not available | not available | Ex-cond_1 |
| DIPSI-C(CCO)NH | not available | not available | Ex-cond_1 |
| HAHB | not available | not available | Ex-cond_1 |
| HCCH-TOCSY | not available | not available | Ex-cond_1 |
| HCCH-COSY | not available | not available | Ex-cond_1 |
| 1H-13C HSQC | not available | not available | Ex-cond_1 |
| 15N-HOHAHA-HSQC | not available | not available | Ex-cond_1 |
| 15N-NOESY-HSQC | not available | not available | Ex-cond_1 |
| 13C-15N NOESY | not available | not available | Ex-cond_1 |
| 13C-13C NOESY | not available | not available | Ex-cond_1 |
Software:
NMRPIPE - data processing, analysis
PIPP - data processing, analysis
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92889 BAJ17933 |
| GB | AAC41981 AAC63361 AAF06162 AAI72840 AAR05448 |
| REF | NP_000544 |
| SP | Q14191 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts