BMRB Entry 6565
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6565
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Assignment of 1H, 13C and 15N resonances of barnacle cement protein Mrcp-20k PubMed: 16132826
Deposition date: 2005-03-22 Original release date: 2005-09-01
Authors: Suzuki, Rintaro; Mori, Youichi; Kamino, Kei; Yamazaki, Toshimasa
Citation: Suzuki, Rintaro; Mori, Youichi; Kamino, Kei; Yamazaki, Toshimasa. "NMR Assignment of the Barnacle Cement Protein Mrcp-20k" J. Biomol. NMR 32, 257-257 (2005).
Assembly members:
Mrcp20k, polymer, 186 residues, Formula weight is not available
Natural source: Common Name: Acorn barnacle Taxonomy ID: 6680 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Megabalanus rosa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Mrcp20k: AMAHEEDGVCNSNAPCYHCD
ANGENCSCNCELFDCEAKKP
DGSYAHPCRRCDANNICKCS
CTAIPCNEDHPCHHCHEEDD
GDTHCHCSCEHSHDHHDDDT
HGECTKKAPCWRCEYNADLK
HDVCGCECSKLPCNDEHPCY
RKEGGVVSCDCKTITCNEDH
PCYHSYEEDGVTKSDCDCEH
SPGPSE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 619 |
| 15N chemical shifts | 172 |
| 1H chemical shifts | 908 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mrcp20k | 1 |
Entities:
Entity 1, Mrcp20k 186 residues - Formula weight is not available
| 1 | ALA | MET | ALA | HIS | GLU | GLU | ASP | GLY | VAL | CYS | ||||
| 2 | ASN | SER | ASN | ALA | PRO | CYS | TYR | HIS | CYS | ASP | ||||
| 3 | ALA | ASN | GLY | GLU | ASN | CYS | SER | CYS | ASN | CYS | ||||
| 4 | GLU | LEU | PHE | ASP | CYS | GLU | ALA | LYS | LYS | PRO | ||||
| 5 | ASP | GLY | SER | TYR | ALA | HIS | PRO | CYS | ARG | ARG | ||||
| 6 | CYS | ASP | ALA | ASN | ASN | ILE | CYS | LYS | CYS | SER | ||||
| 7 | CYS | THR | ALA | ILE | PRO | CYS | ASN | GLU | ASP | HIS | ||||
| 8 | PRO | CYS | HIS | HIS | CYS | HIS | GLU | GLU | ASP | ASP | ||||
| 9 | GLY | ASP | THR | HIS | CYS | HIS | CYS | SER | CYS | GLU | ||||
| 10 | HIS | SER | HIS | ASP | HIS | HIS | ASP | ASP | ASP | THR | ||||
| 11 | HIS | GLY | GLU | CYS | THR | LYS | LYS | ALA | PRO | CYS | ||||
| 12 | TRP | ARG | CYS | GLU | TYR | ASN | ALA | ASP | LEU | LYS | ||||
| 13 | HIS | ASP | VAL | CYS | GLY | CYS | GLU | CYS | SER | LYS | ||||
| 14 | LEU | PRO | CYS | ASN | ASP | GLU | HIS | PRO | CYS | TYR | ||||
| 15 | ARG | LYS | GLU | GLY | GLY | VAL | VAL | SER | CYS | ASP | ||||
| 16 | CYS | LYS | THR | ILE | THR | CYS | ASN | GLU | ASP | HIS | ||||
| 17 | PRO | CYS | TYR | HIS | SER | TYR | GLU | GLU | ASP | GLY | ||||
| 18 | VAL | THR | LYS | SER | ASP | CYS | ASP | CYS | GLU | HIS | ||||
| 19 | SER | PRO | GLY | PRO | SER | GLU |
Samples:
sample_1: Mrcp20k, [U-13C; U-15N], 7 mM; Sodium Phosphate 10 mM; D2O 10%
sample_2: Mrcp20k, [U-13C; U-15N], 7 mM; Sodium Phosphate 10 mM; D2O 99.8%
condition_1: pH*: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | not available | not available | condition_1 |
| 3D HNCO | not available | not available | condition_1 |
| 3D HNCACB | not available | not available | condition_1 |
| 3D CBCA(CO)NH | not available | not available | condition_1 |
| 3D HNCA | not available | not available | condition_1 |
| 3D 15N-separated NOESY-HSQC | not available | not available | condition_1 |
| 3D 13C/15N-separated NOESY-HSQC | not available | not available | condition_1 |
| 1H13C HSQC | not available | not available | condition_1 |
| 3D HCCH-TOCSY | not available | not available | condition_1 |
| 4D 13C/13C-separated HSQC-NOESY-HSQC | not available | not available | condition_1 |
Software:
NMRPipe v2.3 -
SPARKY v3.110 -
NMR spectrometers:
- Bruker DMX 750 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts