BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6601

Title: 1H, 13C, and 15N chemical shift assignments for B. subtilis PrsA PPIase   PubMed: 16516208

Deposition date: 2005-04-19 Original release date: 2006-04-25

Authors: Tossavainen, Helena; Permi, Perttu; Purhonen, Susanna; Sarvas, Matti; Kilpelainen, Ilkka; Seppala, Raili

Citation: Tossavainen, Helena; Permi, Perttu; Purhonen, Susanna; Sarvas, Matti; Kilpelainen, Ilkka; Seppala, Raili. "NMR solution structure and characterization of substrate binding site of the PPIase domain of PrsA protein from Bacillus subtilis"  FEBS Lett. 580, 1822-1826 (2006).

Assembly members:
PrsA PPIase, polymer, 93 residues, 10579 Da.

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PrsA PPIase: GSGKIRASHILVADKKTAEE VEKKLKKGEKFEDLAKEYST DSSASKGGDLGWFAKEGQMD ETFSKAAFKLKTGEVSDPVK TQYGYHIIKKTEE

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts92
1H chemical shifts636

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PrsA PPIase1

Entities:

Entity 1, PrsA PPIase 93 residues - 10579 Da.

1   GLYSERGLYLYSILEARGALASERHISILE
2   LEUVALALAASPLYSLYSTHRALAGLUGLU
3   VALGLULYSLYSLEULYSLYSGLYGLULYS
4   PHEGLUASPLEUALALYSGLUTYRSERTHR
5   ASPSERSERALASERLYSGLYGLYASPLEU
6   GLYTRPPHEALALYSGLUGLYGLNMETASP
7   GLUTHRPHESERLYSALAALAPHELYSLEU
8   LYSTHRGLYGLUVALSERASPPROVALLYS
9   THRGLNTYRGLYTYRHISILEILELYSLYS
10   THRGLUGLU

Samples:

sample_1: PrsA PPIase, [U-13C; U-15N], 0.9 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
iHNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
iHNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
C(CCO)NHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
CBCGCDHDnot availablenot availablenot available
CBCGCGCEHEnot availablenot availablenot available

Software:

Vnmr v6.1c - Fourier transformation

Sparky v3.106 - peak assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA40543 CAA74418 CAB12835
REF NP_388876
SWISS-PROT P24327

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts