BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6622

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for HEWL-SMe

Deposition date: 2005-05-10 Original release date: 2008-07-16

Authors: Schlorb, Christian; Ackermann, Katrin; Richter, Christian; Wirmer, Julia; Schwalbe, Harald

Citation: Schlorb, Christian; Ackermann, Katrin; Richter, Christian; Wirmer, Julia; Schwalbe, Harald. "Heterologous expression of hen egg white lysozyme and resonance assignment of tryptophan side chains in its non-native states"  J. Biomol. NMR 33, 95-104 (2005).

Assembly members:
Hen egg white lysozyme, polymer, 130 residues, 14444.3 Da.

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Hen egg white lysozyme: MKVFGRXELAAAMKRHGLDN YRGYSLGNWVXAAKFESNFN TQATNRNTDGSTDYGILQIN SRWWXNDGRTPGSRNLXNIP XSALLSSDITASVNXAKKIV SDGNGMNAWVAWRNRXKGTD VQAWIRGXRL

Data sets:
Data typeCount
1H chemical shifts402
15N chemical shifts116
13C chemical shifts332

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hewl-sme1

Entities:

Entity 1, hewl-sme 130 residues - 14444.3 Da.

1   METLYSVALPHEGLYARGSMCGLULEUALA
2   ALAALAMETLYSARGHISGLYLEUASPASN
3   TYRARGGLYTYRSERLEUGLYASNTRPVAL
4   SMCALAALALYSPHEGLUSERASNPHEASN
5   THRGLNALATHRASNARGASNTHRASPGLY
6   SERTHRASPTYRGLYILELEUGLNILEASN
7   SERARGTRPTRPSMCASNASPGLYARGTHR
8   PROGLYSERARGASNLEUSMCASNILEPRO
9   SMCSERALALEULEUSERSERASPILETHR
10   ALASERVALASNSMCALALYSLYSILEVAL
11   SERASPGLYASNGLYMETASNALATRPVAL
12   ALATRPARGASNARGSMCLYSGLYTHRASP
13   VALGLNALATRPILEARGGLYSMCARGLEU

Samples:

sample_1: Hen egg white lysozyme, [U-13C; U-15N], 0.5 mM

condition_1: pH: 2.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablecondition_1

Software:

No software information available

NMR spectrometers:

  • BRUKER DRX 700 MHz
  • BRUKER DRX 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts