BMRB Entry 6628
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6628
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Title: Specificity and Mechanism of the Histone Methyltransferase Pr-Set7 PubMed: 15933069
Deposition date: 2005-05-12 Original release date: 2005-11-22
Authors: Xiao, Bing; Jing, Chun; Kelly, Geoff; Walker, Philip; Muskett, Frederick; Frenkiel, Thomas; Martin, Steve; Sarma, Kavitha; Reinberg, Danny; Gamblin, Steven; Wilson, Jonathan
Citation: Xiao, Bing; Jing, Chun; Kelly, Geoff; Walker, Philip; Muskett, Frederick; Frenkiel, Thomas; Martin, Steve; Sarma, Kavitha; Reinberg, Danny; Gamblin, Steven; Wilson, Jonathan. "Specificity and mechanism of the histone methyltransferase Pr-Set7" Genes Dev. 19, 1444-1454 (2005).
Assembly members:
set7/9, polymer, 264 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
set7/9: GPLGSGQYKDNIRHGVCWIY
YPDGGSLVGEVNEDGEMTGE
KIAYVYPDERTALYGKFIDG
EMIEGKLATLMSTEEGRPHF
ELMPGNSVYHFDKSTSSCIS
TNALLPDPYESERVYVAESL
ISSAGEGLFSKVAVGPNTVM
SFYNGVRITHQEVDSRDWAL
NGNTLSLDEETVIDVPEPYN
HVSKYCASLGHKANHSFTPN
CIYDMFVHPRFGPIKCIRTL
RAVEADEELTVAYGYDHSPP
GKSGPEAPEWYQVELKAFQA
TQQK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 418 |
| 15N chemical shifts | 179 |
| 1H chemical shifts | 179 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Set7/9 | 1 |
Entities:
Entity 1, Set7/9 264 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | GLY | GLN | TYR | LYS | ASP | ||||
| 2 | ASN | ILE | ARG | HIS | GLY | VAL | CYS | TRP | ILE | TYR | ||||
| 3 | TYR | PRO | ASP | GLY | GLY | SER | LEU | VAL | GLY | GLU | ||||
| 4 | VAL | ASN | GLU | ASP | GLY | GLU | MET | THR | GLY | GLU | ||||
| 5 | LYS | ILE | ALA | TYR | VAL | TYR | PRO | ASP | GLU | ARG | ||||
| 6 | THR | ALA | LEU | TYR | GLY | LYS | PHE | ILE | ASP | GLY | ||||
| 7 | GLU | MET | ILE | GLU | GLY | LYS | LEU | ALA | THR | LEU | ||||
| 8 | MET | SER | THR | GLU | GLU | GLY | ARG | PRO | HIS | PHE | ||||
| 9 | GLU | LEU | MET | PRO | GLY | ASN | SER | VAL | TYR | HIS | ||||
| 10 | PHE | ASP | LYS | SER | THR | SER | SER | CYS | ILE | SER | ||||
| 11 | THR | ASN | ALA | LEU | LEU | PRO | ASP | PRO | TYR | GLU | ||||
| 12 | SER | GLU | ARG | VAL | TYR | VAL | ALA | GLU | SER | LEU | ||||
| 13 | ILE | SER | SER | ALA | GLY | GLU | GLY | LEU | PHE | SER | ||||
| 14 | LYS | VAL | ALA | VAL | GLY | PRO | ASN | THR | VAL | MET | ||||
| 15 | SER | PHE | TYR | ASN | GLY | VAL | ARG | ILE | THR | HIS | ||||
| 16 | GLN | GLU | VAL | ASP | SER | ARG | ASP | TRP | ALA | LEU | ||||
| 17 | ASN | GLY | ASN | THR | LEU | SER | LEU | ASP | GLU | GLU | ||||
| 18 | THR | VAL | ILE | ASP | VAL | PRO | GLU | PRO | TYR | ASN | ||||
| 19 | HIS | VAL | SER | LYS | TYR | CYS | ALA | SER | LEU | GLY | ||||
| 20 | HIS | LYS | ALA | ASN | HIS | SER | PHE | THR | PRO | ASN | ||||
| 21 | CYS | ILE | TYR | ASP | MET | PHE | VAL | HIS | PRO | ARG | ||||
| 22 | PHE | GLY | PRO | ILE | LYS | CYS | ILE | ARG | THR | LEU | ||||
| 23 | ARG | ALA | VAL | GLU | ALA | ASP | GLU | GLU | LEU | THR | ||||
| 24 | VAL | ALA | TYR | GLY | TYR | ASP | HIS | SER | PRO | PRO | ||||
| 25 | GLY | LYS | SER | GLY | PRO | GLU | ALA | PRO | GLU | TRP | ||||
| 26 | TYR | GLN | VAL | GLU | LEU | LYS | ALA | PHE | GLN | ALA | ||||
| 27 | THR | GLN | GLN | LYS |
Samples:
sample_1: set7/9, [U-98% 13C; U-99% 15N; U-98% 2H], 0.5 mM; Tris 20 mM; TCEP 0.5 mM
conditions_1: ionic strength: 0.1 M; pH: 7.0
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H{15N}-TROSY | sample_1 | not available | conditions_1 |
| TROSY-HNCA | not available | not available | not available |
| TROSY-HN(CO)CA | not available | not available | not available |
| TROSY-HN(CA)CO | not available | not available | not available |
| TROSY-HNCO | not available | not available | not available |
| TROSY-HN(CA)CB | not available | not available | not available |
| TROSY-HN(COCA)CB | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB21808 BAG11355 |
| GB | AAI21056 AAI21057 AAL56579 AAL69901 AAY40937 |
| REF | NP_001244432 NP_085151 XP_002745388 XP_002815202 XP_002916185 |
| SP | Q8WTS6 |
| TPG | DAA20878 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts