BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6646

Title: 1H, 15N and 13C backbone assignment of the firefly luciferase C-terminal 14.3 kDa domain   PubMed: 16222562

Deposition date: 2005-05-24 Original release date: 2005-10-26

Authors: Branchini, Bruce; Gonzalez, Susan; Magyar, Rachelle

Citation: Branchini, Bruce; Gonzalez, Susan; Magyar, Rachelle. "NMR Assignment of the Backbone Resonances of the Firefly Luciferase C-terminal 14.3 kDa Domain"  J. Biomol. NMR 33, 73-73 (2005).

Assembly members:
firefly luciferase C-terminal domain (420-550), polymer, 131 residues, Formula weight is not available

Natural source:   Common Name: common eastern firefly   Taxonomy ID: 7054   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Photinus pyralis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
firefly luciferase C-terminal domain (420-550): SGDIAYWDEDEHFFIVDRLK SLIKYKGYQVAPAELESILL QHPNIFDAGVAGLPDDDAGE LPAAVVVLEHGKTMTEKEIV DYVASQVTTAKKLRGGVVFV DEVPKGLTGKLDARKIREIL IKAKKGGKSKL

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts123
1H chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1firefly luciferase domain residues 420-5501

Entities:

Entity 1, firefly luciferase domain residues 420-550 131 residues - Formula weight is not available

1   SERGLYASPILEALATYRTRPASPGLUASP
2   GLUHISPHEPHEILEVALASPARGLEULYS
3   SERLEUILELYSTYRLYSGLYTYRGLNVAL
4   ALAPROALAGLULEUGLUSERILELEULEU
5   GLNHISPROASNILEPHEASPALAGLYVAL
6   ALAGLYLEUPROASPASPASPALAGLYGLU
7   LEUPROALAALAVALVALVALLEUGLUHIS
8   GLYLYSTHRMETTHRGLULYSGLUILEVAL
9   ASPTYRVALALASERGLNVALTHRTHRALA
10   LYSLYSLEUARGGLYGLYVALVALPHEVAL
11   ASPGLUVALPROLYSGLYLEUTHRGLYLYS
12   LEUASPALAARGLYSILEARGGLUILELEU
13   ILELYSALALYSLYSGLYGLYLYSSERLYS
14   LEU

Samples:

sample_1: firefly luciferase domain residues 420-550, [U-95% 13C; U-95% 15N], 1.2 mM; sodium phosphate 100 mM; sodium azide 0.05%; H2O 92%; D2O 8%

conditions_1: pH: 7.0; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNHAsample_1not availableconditions_1
NOESY-HSQCsample_1not availableconditions_1

Software:

VNMR v6.1C, Varian -

SPARKY, . -

NMR spectrometers:

  • Varian UNITY/INOVA 500 MHz MHz

Related Database Links:

PDB
DBJ BAA93575 BAB32737 BAD00047 BAE33044 BAE33661
EMBL CAA46407 CAA46419 CAA46421 CAA46423 CAA46425
GB AAA03561 AAA29795 AAA66377 AAA72988 AAA88784
SP P08659

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts