BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6707

Title: NMR assignments for apo- and Ca2+ saturated paramecium calmodulin   PubMed: 12450379

Deposition date: 2005-06-24 Original release date: 2013-02-15

Authors: Jaren, Olav; Kranz, James; Sorensen, Brenda; Wand, A.; Shea, Madeline

Citation: Jaren, Olav; Kranz, James; Sorensen, Brenda; Wand, A.; Shea, Madeline. "Calcium-induced conformational switching of Paramecium calmodulin provides evidence for domain coupling."  Biochemistry 41, 14158-14166 (2002).

Assembly members:
PCaM under apo conditions, polymer, 148 residues, Formula weight is not available

Natural source:   Common Name: Paramecium   Taxonomy ID: 5884   Superkingdom: Eukaryota   Kingdom: Protista   Genus/species: Paramecium not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PCaM under apo conditions: AEQLTEEQIAEFKEAFALFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLSLMARKMKEQD SEEELIEAFKVFDRDGNGLI SAAELRHVMTNLGEKLTDDE VDEMIREADIDGDGHINYEE FVRMMVSK

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts144
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1paramecium calmodulin1

Entities:

Entity 1, paramecium calmodulin 148 residues - Formula weight is not available

1   ALAGLUGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHEALALEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUSER
8   LEUMETALAARGLYSMETLYSGLUGLNASP
9   SERGLUGLUGLULEUILEGLUALAPHELYS
10   VALPHEASPARGASPGLYASNGLYLEUILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPASPGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYHISILEASNTYRGLUGLU
15   PHEVALARGMETMETVALSERLYS

Samples:

sample_1: PCaM, [U-13C; U-15N], 1.0 mM; imidazole-d4 10 mM; KCl 25 mM; EGTA 0.05 mM; NaN3 0.01%; D2O 8%

conditions_1: pH: 6.5; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableconditions_1
1H-13C constant time HSQCsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
CTSL-HCANHsample_1not availableconditions_1
15N-edited 3D NOESYsample_1not availableconditions_1

Software:

FELIX, Molecular Simulations Inc. - spectra processing

NMRView - processed spectra examination

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 700 MHz

Related Database Links:

BMRB 7015
PDB
EMBL CAK80966
GB AAA29443 AAB20487 AAB31200 AAT38517
REF XP_001448363
SP P07463

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts