BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6722

Title: Backbone NMR Assignment of low-molecular-weight Protein Tryosin Phosphatase (MPtpA) from Mycobacterium tuberculosis   PubMed: 16258834

Deposition date: 2005-07-01 Original release date: 2005-11-07

Authors: Saxena, Krishna; Elshorst, Bettina; Berk, Holger; Betz, Marco; Grimme, Susanne; Langer, Thomas; Pescatore, Barbara; Schieborr, Ulrich; Vogtherr, Martin; Schwalbe, Harald

Citation: Saxena, Krishna; Elshorst, Bettina; Berk, Holger; Betz, Marco; Grimme, Susanne; Langer, Thomas; Pescatore, Barbara; Schieborr, Ulrich; Vogtherr, Martin; Schwalbe, Harald. "Backbone NMR Assignment of the Low-molecular-weight Protein Tyrosine Phosphatase (MPtpA) from Mycobacterium Tuberculosis"  J. Biomol. NMR 33, 136-136 (2005).

Assembly members:
Low-Molecular-Weight Protein Tyrosine Phosphatase, polymer, 165 residues, Formula weight is not available

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Mycobacterium tuberculosis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Low-Molecular-Weight Protein Tyrosine Phosphatase: GHMSDPLHVTFVCTGNICRS PMAEKMFAQQLRHRGLGDAV RVTSAGTGNWHVGSCADERA AGVLRAHGYPTDHRAAQVGT EHLAADLLVALDRNHARLLR QLGVEAARVRMLRSFDPRSG THALDVEDPYYGDHSDFEEV FAVIESALPGLHDWVDERLA RNGPS

Data sets:
Data typeCount
13C chemical shifts444
15N chemical shifts147
1H chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Low-Molecular-Weight Protein Tyrosin Phosphatase1

Entities:

Entity 1, Low-Molecular-Weight Protein Tyrosin Phosphatase 165 residues - Formula weight is not available

The first two amino acids are due to cloning

1   GLYHISMETSERASPPROLEUHISVALTHR
2   PHEVALCYSTHRGLYASNILECYSARGSER
3   PROMETALAGLULYSMETPHEALAGLNGLN
4   LEUARGHISARGGLYLEUGLYASPALAVAL
5   ARGVALTHRSERALAGLYTHRGLYASNTRP
6   HISVALGLYSERCYSALAASPGLUARGALA
7   ALAGLYVALLEUARGALAHISGLYTYRPRO
8   THRASPHISARGALAALAGLNVALGLYTHR
9   GLUHISLEUALAALAASPLEULEUVALALA
10   LEUASPARGASNHISALAARGLEULEUARG
11   GLNLEUGLYVALGLUALAALAARGVALARG
12   METLEUARGSERPHEASPPROARGSERGLY
13   THRHISALALEUASPVALGLUASPPROTYR
14   TYRGLYASPHISSERASPPHEGLUGLUVAL
15   PHEALAVALILEGLUSERALALEUPROGLY
16   LEUHISASPTRPVALASPGLUARGLEUALA
17   ARGASNGLYPROSER

Samples:

sample_1: Low-Molecular-Weight Protein Tyrosine Phosphatase, [U-13C; U-15N], 0.5 mM; Hepes 25 mM; NaCL 150 mM; DTT 10 mM

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCACOsample_1not availableconditions_1

Software:

CARA v1.3, Rochus Keller, Prof. Kurt Wuthrich - Assignment

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 18533 19361 19388
PDB
DBJ BAH26529 BAL66249 BAQ06307 GAA45923
EMBL CAD97111 CAL72239 CCC27315 CCC44588 CCC64828
GB AAK46577 ABQ74015 ABR06594 ACT24805 AEB03885
REF NP_216750 NP_336763 NP_855907 WP_003411510 WP_003899227
SP P65717 P9WIA0 P9WIA1

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts