BMRB Entry 6928
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6928
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Title: 1H, 13C, and 15N NMR Assignment of the Rep Protein Nuclease Domain from the Porcine Circovirus PCV2 PubMed: 16636755
Deposition date: 2005-12-21 Original release date: 2006-05-02
Authors: Vega, Susana; Campos-Olivas, Ramon
Citation: Rocha, Susana; Campos-Olivas, Ramon. "1H, 13C, and 15N NMR Assignment of the Rep Protein Nuclease Domain from the Porcine Circovirus PCV2" J. Biomol. NMR 36, 25-25 (2006).
Assembly members:
N-terminal DNA binding domain of Rep protein from the circovirus PCV2, polymer, 115 residues, 13251.9 Da.
Natural source: Common Name: Porcine circovirus 2 Taxonomy ID: 46221 Superkingdom: Viruses Kingdom: not available Genus/species: Circovirus Porcine circovirus 2
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
N-terminal DNA binding domain of Rep protein from the circovirus PCV2: PSKKNGRSGPQPHKRWVFTL
NNPSEDERKKIRDLPISLFD
YFIVGEEGNEEGRTPHLQGF
ANFVKKQTFNKVKWYLGARC
HIEKAKGTDQQNKEYCSKEG
NLLMECGAPRSQGQR
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 498 |
15N chemical shifts | 125 |
1H chemical shifts | 752 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PCV2 | 1 |
Entities:
Entity 1, PCV2 115 residues - 13251.9 Da.
1 | PRO | SER | LYS | LYS | ASN | GLY | ARG | SER | GLY | PRO | ||||
2 | GLN | PRO | HIS | LYS | ARG | TRP | VAL | PHE | THR | LEU | ||||
3 | ASN | ASN | PRO | SER | GLU | ASP | GLU | ARG | LYS | LYS | ||||
4 | ILE | ARG | ASP | LEU | PRO | ILE | SER | LEU | PHE | ASP | ||||
5 | TYR | PHE | ILE | VAL | GLY | GLU | GLU | GLY | ASN | GLU | ||||
6 | GLU | GLY | ARG | THR | PRO | HIS | LEU | GLN | GLY | PHE | ||||
7 | ALA | ASN | PHE | VAL | LYS | LYS | GLN | THR | PHE | ASN | ||||
8 | LYS | VAL | LYS | TRP | TYR | LEU | GLY | ALA | ARG | CYS | ||||
9 | HIS | ILE | GLU | LYS | ALA | LYS | GLY | THR | ASP | GLN | ||||
10 | GLN | ASN | LYS | GLU | TYR | CYS | SER | LYS | GLU | GLY | ||||
11 | ASN | LEU | LEU | MET | GLU | CYS | GLY | ALA | PRO | ARG | ||||
12 | SER | GLN | GLY | GLN | ARG |
Samples:
sample_1: Rep PCV2 2-116, [U-10% 13C; U-15N], 0.8 M; Sodium phosphate 20 mM; NaCl 100 mM; DTT 1 mM; D2O 8%
sample_2: Rep PCV2 2-116, [U-10% 13C; U-15N], 0.8 M; Sodium phosphate 20 mM; NaCl 100 mM; DTT 1 mM; D2O 100%
sample_3: Rep PCV2 2-116, [U-13C; U-15N], 0.8 M; Sodium phosphate 20 mM; NaCl 100 mM; DTT 1 mM; D2O 8%
sample_4: Rep PCV2 2-116, [U-13C; U-15N], 0.8 M; Sodium phosphate 20 mM; NaCl 100 mM; DTT 1 mM; D2O 100%
conditions_1: ionic strength: 0.264 M; pH: 6.6; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D_1H15N_HSQC | not available | not available | conditions_1 |
2D_1H15N_HSQC_long_range | not available | not available | conditions_1 |
2D_1H13C_HSQC/HMQC | not available | not available | conditions_1 |
2D_HNCOCG_arom | not available | not available | conditions_1 |
2D_HNCO_arom | not available | not available | conditions_1 |
3D_HNCO | not available | not available | conditions_1 |
3D_HNCACB | not available | not available | conditions_1 |
3D_CBCA_(CO)_NH | not available | not available | conditions_1 |
3D_HNHA | not available | not available | conditions_1 |
3D_HNHB | not available | not available | conditions_1 |
3D_15N_edited_NOESY | not available | not available | conditions_1 |
4D_15N,_13C_NOESY | not available | not available | conditions_1 |
4D_13C,_13C_NOESY | not available | not available | conditions_1 |
3D_HCACO | not available | not available | conditions_1 |
3D_CC_(CO)_NH | not available | not available | conditions_1 |
HCCH_TOCSY | not available | not available | conditions_1 |
3D_15N_edited_TOCSY | not available | not available | conditions_1 |
Software:
NMRView v5.0.20 -
NMRPipe v97.027.12.56 -
NMR spectrometers:
- Bruker AMX Avance 600 MHz
- Bruker AMX Avance 700 MHz
Related Database Links:
PDB | |
DBJ | BAA88133 BAB69432 BAB69437 BAB69441 BAG12761 |
EMBL | CAA11157 CAC41083 CAC41084 CAC41085 CAF25171 |
GB | AAC35298 AAC35309 AAC35320 AAC35330 AAC59462 |
REF | NP_937956 |
SP | Q8BB16 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts