BMRB Entry 6930
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6930
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Title: NMR assignments of SNF RBD1 PubMed: 19727396
Deposition date: 2006-01-03 Original release date: 2009-10-20
Authors: Liu, Cong; Cui, Gaofeng; Jin, Changwen; Xia, Bin
Citation: Hu, Jicheng; Cui, Gaofeng; Li, Congmin; Liu, Cong; Shang, Erchang; Lai, Luhua; Jin, Changwen; Wang, Jiwu; Xia, Bin. "Structure and Novel Functional Mechanism of Drosophila SNF in Sex-Lethal Splicing" PLoS One 4, 6890-. (2009).
Assembly members:
snRNP protein, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
snRNP protein: MEMLPNQTIYINNLNEKIKK
EELKKSLYAIFSQFGQILDI
VALKTLKMRGQAFVIFKEIG
SASNALRTMQGFPFYDKPMQ
IAYSKSDSDIVAKIKGTFKE
RPKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 457 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 762 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SNF RBD1 | 1 |
Entities:
Entity 1, SNF RBD1 104 residues - Formula weight is not available
| 1 | MET | GLU | MET | LEU | PRO | ASN | GLN | THR | ILE | TYR | ||||
| 2 | ILE | ASN | ASN | LEU | ASN | GLU | LYS | ILE | LYS | LYS | ||||
| 3 | GLU | GLU | LEU | LYS | LYS | SER | LEU | TYR | ALA | ILE | ||||
| 4 | PHE | SER | GLN | PHE | GLY | GLN | ILE | LEU | ASP | ILE | ||||
| 5 | VAL | ALA | LEU | LYS | THR | LEU | LYS | MET | ARG | GLY | ||||
| 6 | GLN | ALA | PHE | VAL | ILE | PHE | LYS | GLU | ILE | GLY | ||||
| 7 | SER | ALA | SER | ASN | ALA | LEU | ARG | THR | MET | GLN | ||||
| 8 | GLY | PHE | PRO | PHE | TYR | ASP | LYS | PRO | MET | GLN | ||||
| 9 | ILE | ALA | TYR | SER | LYS | SER | ASP | SER | ASP | ILE | ||||
| 10 | VAL | ALA | LYS | ILE | LYS | GLY | THR | PHE | LYS | GLU | ||||
| 11 | ARG | PRO | LYS | LYS |
Samples:
sample_1: SNF RBD1, [U-15N; U-13C], 0.4 ± 0.05 mM; PBS 50 mM; NaCl 50 mM; H2O 90%; D2Ol 10%
conditions_1: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | not available | conditions_1 |
| 2D 1H-13C HSQC | sample_1 | not available | conditions_1 |
| 3D HNCACB | sample_1 | not available | conditions_1 |
| 3D CBCA(CO)NH | sample_1 | not available | conditions_1 |
| 3D HNCO | sample_1 | not available | conditions_1 |
| 3D HNCA | sample_1 | not available | conditions_1 |
| 3D HCCH-TOCSY | sample_1 | not available | conditions_1 |
| 3D HCCH-COSY | sample_1 | not available | conditions_1 |
| 3D (H)CCH-COSY | sample_1 | not available | conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | not available | conditions_1 |
| 3D 15N-separated NOESY | sample_1 | not available | conditions_1 |
| 3D 13C-separated NOESY | sample_1 | not available | conditions_1 |
Software:
NMRView v5 -
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB | |
| GB | AAA28441 AAA28903 AAF46017 AAL29039 ACL84772 |
| REF | NP_511045 XP_002036935 XP_002076873 |
| SP | P43332 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts