BMRB Entry 6984
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6984
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Title: 1H, 13C, and 15N Chemical Shift Assignments of the cAMP-Binding Domain A of the PKA Regulatory Subunit PubMed: 17016670
Deposition date: 2006-02-14 Original release date: 2006-12-22
Authors: Esposito, Veronica; Sjoberg, Tim; Das, Rahul; Brown, Simon; Taylor, Susan; Melacini, Giuseppe
Citation: Esposito, Veronica; Sjoberg, Tim; Das, Rahul; Brown, Simon; Taylor, Susan; Melacini, Giuseppe. "NMR assignment of the cAMP-binding domain A of the PKA regulatory subunit" J. Biomol. NMR 36, 64-64 (2006).
Assembly members:
PKA regulatory subunit, polymer, 126 residues, 14156 Da.
CMP, non-polymer, 329.206 Da.
Natural source: Common Name: Bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos Taurus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PKA regulatory subunit: DYKTMAALAKAIEKNVLFSH
LDDNERSDIFDAMFPVSFIA
GETVIQQGDEGDNFYVIDQG
EMDVYVNNEWATSVGEGGSF
GELALIYGTPRAATVKAKTN
VKLWGIDRDSYRRILMGSTL
RKRKMY
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 476 |
15N chemical shifts | 130 |
1H chemical shifts | 790 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | cAMP Binding Domain A | 1 |
2 | cAMP | 2 |
Entities:
Entity 1, cAMP Binding Domain A 126 residues - 14156 Da.
1 | ASP | TYR | LYS | THR | MET | ALA | ALA | LEU | ALA | LYS | ||||
2 | ALA | ILE | GLU | LYS | ASN | VAL | LEU | PHE | SER | HIS | ||||
3 | LEU | ASP | ASP | ASN | GLU | ARG | SER | ASP | ILE | PHE | ||||
4 | ASP | ALA | MET | PHE | PRO | VAL | SER | PHE | ILE | ALA | ||||
5 | GLY | GLU | THR | VAL | ILE | GLN | GLN | GLY | ASP | GLU | ||||
6 | GLY | ASP | ASN | PHE | TYR | VAL | ILE | ASP | GLN | GLY | ||||
7 | GLU | MET | ASP | VAL | TYR | VAL | ASN | ASN | GLU | TRP | ||||
8 | ALA | THR | SER | VAL | GLY | GLU | GLY | GLY | SER | PHE | ||||
9 | GLY | GLU | LEU | ALA | LEU | ILE | TYR | GLY | THR | PRO | ||||
10 | ARG | ALA | ALA | THR | VAL | LYS | ALA | LYS | THR | ASN | ||||
11 | VAL | LYS | LEU | TRP | GLY | ILE | ASP | ARG | ASP | SER | ||||
12 | TYR | ARG | ARG | ILE | LEU | MET | GLY | SER | THR | LEU | ||||
13 | ARG | LYS | ARG | LYS | MET | TYR |
Entity 2, cAMP - C10 H12 N5 O6 P - 329.206 Da.
1 | CMP |
Samples:
sample_1: R1a 119-244, [U-13C; U-15N], 0.5 mM; cAMP 1 mM; KH2PO4 20 mM; NaCl 100 mM; 1:1 mixture of Arg:Glu 50 mM; D2O 5%
conditions_1: pH: 6.5; temperature: 309 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
HNCA | sample_1 | not available | conditions_1 |
HN(CO)CA | sample_1 | not available | conditions_1 |
HNCACB | sample_1 | not available | conditions_1 |
CBCA(CO)NH | sample_1 | not available | conditions_1 |
HBHANH | sample_1 | not available | conditions_1 |
HBHA(CO)NH | sample_1 | not available | conditions_1 |
HNCO | sample_1 | not available | conditions_1 |
15N TOCSY-HSQC | sample_1 | not available | conditions_1 |
15N NOESY-HSQC | sample_1 | not available | conditions_1 |
15N HSQC-NOESY-15N HSQC | sample_1 | not available | conditions_1 |
13C HSQC-NOESY-15N HSQC | sample_1 | not available | conditions_1 |
HCcH-COSY | sample_1 | not available | conditions_1 |
HCcH-TOCSY | sample_1 | not available | conditions_1 |
hCCG-TOCSY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
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