BMRB Entry 7004
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7004
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Rhodopseudomonas palustrus protein Rpa0253. Northeast structural genomics target RpR3.
Deposition date: 2006-02-23 Original release date: 2006-03-29
Authors: Ramelot, Theresa; Kennedy, Michael
Citation: Ramelot, Theresa; Kennedy, Michael. "NMR structure of Rpa0253 from Rhodopseudomonas paulstris" The BMRB entry is the only known published source for the data..
Assembly members:
Rpa0253, polymer, 145 residues, Formula weight is not available
Natural source: Common Name: Rhodopseudomonas palustris Taxonomy ID: 1076 Superkingdom: Eubacteria Kingdom: not available Genus/species: Rhodopseudomonas palustris
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Rpa0253: VAYWLVKSEPSVWSWDQQVA
KGAAGEAWTGVRNHSAKLHM
VAMRRGDRAFYYHSNEGKEI
VGIAEIIREAYPDPTDASGK
FVCVDIKADKPLKTPVTLAA
VKAEPRLADMALMKYSRLSV
QPVTAEEWKLVCKMGGLLEH
HHHHH
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 625 |
| 15N chemical shifts | 150 |
| 1H chemical shifts | 1014 |
| coupling constants | 77 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rpa0253 monomer | 1 |
Entities:
Entity 1, Rpa0253 monomer 145 residues - Formula weight is not available
| 1 | VAL | ALA | TYR | TRP | LEU | VAL | LYS | SER | GLU | PRO | ||||
| 2 | SER | VAL | TRP | SER | TRP | ASP | GLN | GLN | VAL | ALA | ||||
| 3 | LYS | GLY | ALA | ALA | GLY | GLU | ALA | TRP | THR | GLY | ||||
| 4 | VAL | ARG | ASN | HIS | SER | ALA | LYS | LEU | HIS | MET | ||||
| 5 | VAL | ALA | MET | ARG | ARG | GLY | ASP | ARG | ALA | PHE | ||||
| 6 | TYR | TYR | HIS | SER | ASN | GLU | GLY | LYS | GLU | ILE | ||||
| 7 | VAL | GLY | ILE | ALA | GLU | ILE | ILE | ARG | GLU | ALA | ||||
| 8 | TYR | PRO | ASP | PRO | THR | ASP | ALA | SER | GLY | LYS | ||||
| 9 | PHE | VAL | CYS | VAL | ASP | ILE | LYS | ALA | ASP | LYS | ||||
| 10 | PRO | LEU | LYS | THR | PRO | VAL | THR | LEU | ALA | ALA | ||||
| 11 | VAL | LYS | ALA | GLU | PRO | ARG | LEU | ALA | ASP | MET | ||||
| 12 | ALA | LEU | MET | LYS | TYR | SER | ARG | LEU | SER | VAL | ||||
| 13 | GLN | PRO | VAL | THR | ALA | GLU | GLU | TRP | LYS | LEU | ||||
| 14 | VAL | CYS | LYS | MET | GLY | GLY | LEU | LEU | GLU | HIS | ||||
| 15 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: Rpa0253, [U-100% 13C; U-100% 15N], 0.5 mM; MES 20 mM; NaCl 5 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%
conditions_1: ionic strength: 100 mM; pH: 6.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 799.7 MHz
- Varian Inova 600.2 MHz
Related Database Links:
| PDB | |
| EMBL | CAE25697 |
| GB | ACE98814 ADU42022 |
| REF | NP_945606 WP_011155821 WP_012494016 WP_013500221 YP_001989290 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts