BMRB Entry 7021
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7021
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Title: Backbone dynamics and domain motions of the Mip protein from legionella pneumophilia in solution PubMed: 17014083
Deposition date: 2006-03-15 Original release date: 2011-08-11
Authors: Horstmann, Martin; Ehses, Philipp; Schweimer, Kristian; Kamphausen, Thilo; Fischer, Gunter; Steinert, Michael; Hacker, org; osch, Paul; Faber, Cornelius
Citation: Horstmann, Martin; Ehses, Philipp; Schweimer, Kristian; Steinert, Michael; Kamphausen, Thilo; Fischer, Gunter; Hacker, Jorg; Roesch, Paul; Faber, Cornelius. "Domain Motions of the Mip Protein from Legionella pneumophila" Biochemistry 45, 12303-12311 (2006).
Assembly members:
LpMip, polymer, 213 residues, Formula weight is not available
Natural source: Common Name: Legionella pneumophila Taxonomy ID: 446 Superkingdom: Eubacteria Kingdom: not available Genus/species: Legionella L. pneumophila
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
LpMip: ATDATSLATDKDKLSYSIGA
DLGKNFKNQGIDVNPEAMAK
GMQDAMSGAQLALTEQQMKD
VLNKFQKDLMAKRTAEFNKK
ADENKVKGEAFLTENKNKPG
VVVLPSGLQYKVINSGNGVK
PGKSDTVTVEYTGRLIDGTV
FDSTEKTGKPATFQVSQVIP
GWTEALQLMPAGSTWEIYVP
SGLAYGPRSVGGPIGPNETL
IFKIHLISVKKSS
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 663 |
| 15N chemical shifts | 178 |
| 1H chemical shifts | 969 |
| T1 relaxation values | 161 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LpMip is a homodimer | 1 |
| 2 | LpMip is a homodimer | 1 |
Entities:
Entity 1, LpMip is a homodimer 213 residues - Formula weight is not available
| 1 | ALA | THR | ASP | ALA | THR | SER | LEU | ALA | THR | ASP | ||||
| 2 | LYS | ASP | LYS | LEU | SER | TYR | SER | ILE | GLY | ALA | ||||
| 3 | ASP | LEU | GLY | LYS | ASN | PHE | LYS | ASN | GLN | GLY | ||||
| 4 | ILE | ASP | VAL | ASN | PRO | GLU | ALA | MET | ALA | LYS | ||||
| 5 | GLY | MET | GLN | ASP | ALA | MET | SER | GLY | ALA | GLN | ||||
| 6 | LEU | ALA | LEU | THR | GLU | GLN | GLN | MET | LYS | ASP | ||||
| 7 | VAL | LEU | ASN | LYS | PHE | GLN | LYS | ASP | LEU | MET | ||||
| 8 | ALA | LYS | ARG | THR | ALA | GLU | PHE | ASN | LYS | LYS | ||||
| 9 | ALA | ASP | GLU | ASN | LYS | VAL | LYS | GLY | GLU | ALA | ||||
| 10 | PHE | LEU | THR | GLU | ASN | LYS | ASN | LYS | PRO | GLY | ||||
| 11 | VAL | VAL | VAL | LEU | PRO | SER | GLY | LEU | GLN | TYR | ||||
| 12 | LYS | VAL | ILE | ASN | SER | GLY | ASN | GLY | VAL | LYS | ||||
| 13 | PRO | GLY | LYS | SER | ASP | THR | VAL | THR | VAL | GLU | ||||
| 14 | TYR | THR | GLY | ARG | LEU | ILE | ASP | GLY | THR | VAL | ||||
| 15 | PHE | ASP | SER | THR | GLU | LYS | THR | GLY | LYS | PRO | ||||
| 16 | ALA | THR | PHE | GLN | VAL | SER | GLN | VAL | ILE | PRO | ||||
| 17 | GLY | TRP | THR | GLU | ALA | LEU | GLN | LEU | MET | PRO | ||||
| 18 | ALA | GLY | SER | THR | TRP | GLU | ILE | TYR | VAL | PRO | ||||
| 19 | SER | GLY | LEU | ALA | TYR | GLY | PRO | ARG | SER | VAL | ||||
| 20 | GLY | GLY | PRO | ILE | GLY | PRO | ASN | GLU | THR | LEU | ||||
| 21 | ILE | PHE | LYS | ILE | HIS | LEU | ILE | SER | VAL | LYS | ||||
| 22 | LYS | SER | SER |
Samples:
sample_1: LpMip, [U-13C; U-15N], 0.9 mM
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | not available |
| CBCA(CO)NH | sample_1 | not available | not available |
| HNCO | sample_1 | not available | not available |
| HNCACB | sample_1 | not available | not available |
| HNCA | sample_1 | not available | not available |
| HBHA(CO)NH | sample_1 | not available | not available |
| C(CO)NH | sample_1 | not available | not available |
| HCCHTOCSY | sample_1 | not available | not available |
| 15N NOESY | sample_1 | not available | not available |
| 13C NOESY | sample_1 | not available | not available |
| 15N 15N NOESY | sample_1 | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts